July 7, 2019  |  

Genomic organization of the zebrafish (Danio rerio) T cell receptor alpha/delta locus and analysis of expressed products.

Authors: Seelye, Stacie L and Chen, Patricia L and Deiss, Thaddeus C and Criscitiello, Michael F

In testing the hypothesis that all jawed vertebrate classes employ immunoglobulin heavy chain V (IgHV) gene segments in their T cell receptor (TCR)d encoding loci, we found that some basic characterization was required of zebrafish TCRd. We began by annotating and characterizing the TCRa/d locus of Danio rerio based on the most recent genome assembly, GRCz10. We identified a total of 141 theoretically functional V segments which we grouped into 41 families based upon 70 % nucleotide identity. This number represents the second greatest count of apparently functional V genes thus far described in an antigen receptor locus with the exception of cattle TCRa/d. Cloning, relative quantitative PCR, and deep sequencing results corroborate that zebrafish do express TCRd, but these data suggest only at extremely low levels and in limited diversity in the spleens of the adult fish. While we found no evidence for IgH-TCRd rearrangements in this fish, by determining the locus organization we were able to suggest how the evolution of the teleost a/d locus could have lost IgHVs that exist in sharks and frogs. We also found evidence of surprisingly low TCRd expression and repertoire diversity in this species.

Journal: Immunogenetics
DOI: 10.1007/s00251-016-0904-3
Year: 2016

Read publication

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.