July 19, 2019  |  

Full-length mitochondrial-DNA sequencing on the PacBio RSII.

Authors: Vossen, Rolf H A M and Buermans, Henk P J

Conventional mitochondrial-DNA (MT DNA) sequencing approaches use Sanger sequencing of 20-40 partially overlapping PCR fragments per individual, which is a time- and resource-consuming process. We have developed a high-throughput, accurate, fast, and cost-effective human MT DNA sequencing approach. In this setup we first generate long-range PCR products for two partially overlapping 7.7 and 9.2 kb MT DNA-specific amplicons, add sample-specific barcodes, and sequence these on the PacBio RSII system to obtain full-length MT DNA sequences for genotyping/haplotyping purposes.

Journal: Methods in molecular biology
DOI: 10.1007/978-1-4939-6442-0_12
Year: 2016

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