July 7, 2019  |  

Epigenetic origin of evolutionary novel centromeres.

Authors: Tolomeo, Doron and Capozzi, Oronzo and Stanyon, Roscoe R and Archidiacono, Nicoletta and D'Addabbo, Pietro and Catacchio, Claudia R and Purgato, Stefania and Perini, Giovanni and Schempp, Werner and Huddleston, John and Malig, Maika and Eichler, Evan E and Rocchi, Mariano

Most evolutionary new centromeres (ENC) are composed of large arrays of satellite DNA and surrounded by segmental duplications. However, the hypothesis is that ENCs are seeded in an anonymous sequence and only over time have acquired the complexity of "normal" centromeres. Up to now evidence to test this hypothesis was lacking. We recently discovered that the well-known polymorphism of orangutan chromosome 12 was due to the presence of an ENC. We sequenced the genome of an orangutan homozygous for the ENC, and we focused our analysis on the comparison of the ENC domain with respect to its wild type counterpart. No significant variations were found. This finding is the first clear evidence that ENC seedings are epigenetic in nature. The compaction of the ENC domain was found significantly higher than the corresponding WT region and, interestingly, the expression of the only gene embedded in the region was significantly repressed.

Journal: Scientific reports
DOI: 10.1038/srep41980
Year: 2017

Read Publication

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.