July 7, 2019  |  

Efficient, cost-effective, high-throughput, Multilocus Sequencing Typing (MLST) method, NGMLST, and the analytical software program MLSTEZ.

Authors: Chen, Yuan and Perfect, John R

Multilocus sequence typing (MLST) has become the preferred method for genotyping many biological species. It can be used to identify major phylogenetic clades, molecular groups, or subpopulations of a species, as well as individual strains or clones. However, conventional MLST is costly and time consuming, which limits its power for genotyping large numbers of samples. Here, we describe a new MLST method that uses next-generation sequencing, a multiplexing protocol, and appropriate analytical software to provide accurate, rapid, and economical MLST genotyping of 96 or more isolates in a single assay.

Journal: Methods in molecular biology
DOI: 10.1007/978-1-4939-6442-0_14
Year: 2016

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