July 7, 2019  |  

Comparative whole genome analysis of three consecutive Salmonella diarizonae isolates.

Authors: Gerlach, Roman G and Walter, Steffi and McClelland, Michael and Schmidt, Christiane and Steglich, Matthias and Prager, Rita and Bender, Jennifer K and Fuchs, Stephan and Schoerner, Christoph and Rabsch, Wolfgang and Lang, Werner and Jantsch, Jonathan

Infections of very young children or immunocompromised people with Salmonella of higher subspecies are a well-known phenomenon often associated with contact to cold-blooded animals. We describe the molecular characterization of three S. enterica subsp. diarizonae strains, isolated consecutively over a period of several months from a hospital patient suffering from diarrhea and sepsis with fatal outcome. With the initial isolate the first complete genome sequence of a member of subsp. diarizonae is provided and based on this reference we revealed the genomic differences between the three isolates by use of next-generation sequencing and confirmed by phenotypical tests. Genome comparisons revealed mutations within gpt, hfq and purK in the first isolate as a sign of clonal variation rather than host-directed evolution. Furthermore, our work demonstrates that S. enterica subsp. diarizonae possess, besides a conserved set of known Salmonella Pathogenicity Islands, a variable portfolio of additional genomic islands of unknown function. Copyright © 2017 Elsevier GmbH. All rights reserved.

Journal: International journal of medical microbiology
DOI: 10.1016/j.ijmm.2017.09.001
Year: 2017

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