April 21, 2020  |  

A draft genome for Spatholobus suberectus.

Authors: Qin, Shuangshuang and Wu, Lingqing and Wei, Kunhua and Liang, Ying and Song, Zhijun and Zhou, Xiaolei and Wang, Shuo and Li, Mingjie and Wu, Qinghua and Zhang, Kaijian and Hui, Yuanyuan and Wang, Shuying and Miao, Jianhua and Zhang, Zhongyi

Spatholobus suberectus Dunn (S. suberectus), which belongs to the Leguminosae, is an important medicinal plant in China. Owing to its long growth cycle and increased use in human medicine, wild resources of S. suberectus have decreased rapidly and may be on the verge of extinction. De novo assembly of the whole S. suberectus genome provides us a critical potential resource towards biosynthesis of the main bioactive components and seed development regulation mechanism of this plant. Utilizing several sequencing technologies such as Illumina HiSeq X Ten, single-molecule real-time sequencing, 10x Genomics, as well as new assembly techniques such as FALCON and chromatin interaction mapping (Hi-C), we assembled a chromosome-scale genome about 798?Mb in size. In total, 748?Mb (93.73%) of the contig sequences were anchored onto nine chromosomes with the longest scaffold being 103.57?Mb. Further annotation analyses predicted 31,634 protein-coding genes, of which 93.9% have been functionally annotated. All data generated in this study is available in public databases.

Journal: Scientific data
DOI: 10.1038/s41597-019-0110-x
Year: 2019

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