Multiplexing Kits

96 Barcodes, 96 Samples

Note: Compatible with SMRTbell Express Template Prep Kit 2.0. Requires Sequel II or IIe System with ICS v10.1.0 or newer.

• Resource: Plate Layout (Excel)
• Resource: Barcode Sequences (FASTA)
• Product insert: SMRTbell Barcoded Adapter Plate 3.0 (96 barcodes, 96 samples)
• SDS: SMRTbell Barcoded Adapter Plate 3.0 (96 barcodes, 96 samples)

Plate contains 384 M13 primer pairs sufficient for 1 sample per primer pair. The 384 primer pairs include all combinations of 24 forward and 16 reverse barcoded primers. Primers can be used in PCR to add barcodes for sample multiplexing

384 Primer Pairs, 384 Samples

Note: Sequel, Sequel II and Sequel IIe Systems compatible.

• Resource: Plate Layout and Primer Sequences (Excel)
• Resource: Primer Sequences (FASTA)
• Product insert: Barcoded M13 Primer Plate (384 primer pairs, 384 samples)
• SDS: Barcoded M13 Primer Plate (384 primer pairs, 384 samples)

The Barcoded Overhang Adapter Kit – 8A/8B are used in conjuntion with the SMRTbell Express Template Prep Kit 2.0 (PN 100-938-900) for multiplexed microbial multiplexing sequencing. The kits can be combined to perform up to 16-plex of pooled microbial genomes. For the Sequel System, we recommend pooling up to 30 MB of total microbial genomes per SMRT Cell to obtain highly contiguous microbial genome assemblies, and up to 40 MB for advanced users. For Sequel II and Sequel IIe Systems, up to 48 microbial genomes can be pooled together per SMRT Cell 8M using the 16 barcodes from this kit and/or supplementing with customer oligos from any oligo synthesis provider. The specific barcodes contained in this kit are as follows for the respective kits: 8A: bc1001, bc1002, bc1003, bc1008, bc1009, bc1010, bc1011, bc1012. 8B: bc1015, bc1016, bc1017, bc1018, bc1019, bc1020, bc1021, bc1022.

Each kit contains 8 barcoded overhang adapters with sufficient reagents to perform 6 ligation reactions for each barcode to support multiplexed microbial genome sequencing, or 12 reactions for amplicon sequencing.

Note: Compatible with all Sequel, Sequel II and Sequel IIe Systems

• Resource: Procedure & Checklist - Preparing Multiplexed Microbial Libraries Using SMRTbell Express Template Prep Kit 2.0
• Resource: Express Microbial Multiplexing Calculator
• Resource: Application Note: Microbial Multiplexing Workflow on the Sequel System using SMRTbell Express Template Prep Kit 2.0
• Resource: Procedure & Checklist - Preparing SMRTbell Libraries using PacBio Barcoded Overhang Adapters for Multiplexing Amplicons
• Product insert: Barcoded Overhang Adapter Kit 8A
• Product insert: Barcoded Overhang Adapter Kit 8B
• SDS: Barcoded Overhang Adapter Kit 8A
• SDS: Barcoded Overhang Adapter Kit 8B

Barcoded Universal F/R Primers Plate – 96 v2 is a 96-well plate containing barcoded primers. Each well on the plate contains a primer pair consisting of forward and reverse primers having the same barcode. Each barcode is present in only one well for a total of 96 different barcodes on the plate. Each primer has a universal sequence on the 3' end to allow for incorporation of barcodes into templates through a two-step PCR strategy. The Barcoded Universal F/R Primers Plate - 96 v2 can be used with SMRTbell Template Prep Kit 1.0 or Express Template Prep Kit 2.0.

Reagent quantities support a single use of each of the 96 primers pairs in library preparations.

Note: Compatible with all PacBio Systems

• Resource: Procedure & Checklist - Preparing SMRTbell Libraries using PacBio Barcoded Universal Primers for Multiplexing Amplicons
• Product insert: Barcoded Universal F/R Primers Plate -96 v2
• SDS: Barcoded Universal F/R Primers Plate -96 v2

The SMRTbell Barcoded Adapter Complete Prep Kit -96 includes the SMRTbell Barcoded Adapter Prep Kit (PN: 100-465-800) and Barcoded Adapter Plate – 96 (PN: 100-466-000). These adapters are ligated directly to amplicons and pooled for damage repair using the SMRTbell Damage Repair Kit - SPv3 (PN:100-992-200, sold separately) prior to sequencing. This kit is based on the SMRTbell Template Prep Kit 1.0 workflow. For Sequel II and Sequel IIe Systems, suggested barcode designs for barcoded adapters which are compatible with the SMRTbell Express Template Preparation Kit 2.0 are available and can be ordered from any customer oligo provider.

Reagent quantities support a single use of each of the 96 primers pairs in library preparations.

Note: Compatible with Sequel System Kit components also sold separately.

• Resource: Procedure & Checklist - Amplicon Template Preparation and Sequencing
• Product insert: SMRTbell Barcoded Adapter Prep Kit
• Product insert: Barcoded Adapter Plate – 96
• SDS: SMRTbell Barcoded Adapter Prep Kit
• SDS: Barcoded Adapter Plate – 96

The SMRTbell Damage Repair Kit-SPv3 contains reagent supplies to perform DNA Damage Repair, Exonuclease III and VII treatments, and primer annealing of SMRTbell libraries made with SMRTbell Template Prep Kit 1.0 using Sequencing primer v3 for insert sizes ranging from 500 bp to over 20 kb. Recommended for use with the SMRTbell Barcoded Adapter Complete Prep Kit (PN: 100-514-900) to generate primer-annealed SMRTbell libraries ready for polymerase binding. Can also be used for extra DNA damage repair of template.

Reagent quantities support 10 library preparations.

Note: Compatible with Sequel SystemSequencing Primer v3 (PN: 100-970-100) also sold separately.

• Resource: Procedure & Checklist - Amplicon Template Preparation and Sequencing
• Product insert: SMRTbell Damage Repair Kit
• Product insert: Sequencing Primer v3
• SDS: SMRTbell Damage Repair Kit
• SDS: Sequencing Primer v3