X

Quality Statement

Pacific Biosciences is committed to providing high-quality products that meet customer expectations and comply with regulations. We will achieve these goals by adhering to and maintaining an effective quality-management system designed to ensure product quality, performance, and safety.

X

Image Use Agreement

By downloading, copying, or making any use of the images located on this website (“Site”) you acknowledge that you have read and understand, and agree to, the terms of this Image Usage Agreement, as well as the terms provided on the Legal Notices webpage, which together govern your use of the images as provided below. If you do not agree to such terms, do not download, copy or use the images in any way, unless you have written permission signed by an authorized Pacific Biosciences representative.

Subject to the terms of this Agreement and the terms provided on the Legal Notices webpage (to the extent they do not conflict with the terms of this Agreement), you may use the images on the Site solely for (a) editorial use by press and/or industry analysts, (b) in connection with a normal, peer-reviewed, scientific publication, book or presentation, or the like. You may not alter or modify any image, in whole or in part, for any reason. You may not use any image in a manner that misrepresents the associated Pacific Biosciences product, service or technology or any associated characteristics, data, or properties thereof. You also may not use any image in a manner that denotes some representation or warranty (express, implied or statutory) from Pacific Biosciences of the product, service or technology. The rights granted by this Agreement are personal to you and are not transferable by you to another party.

You, and not Pacific Biosciences, are responsible for your use of the images. You acknowledge and agree that any misuse of the images or breach of this Agreement will cause Pacific Biosciences irreparable harm. Pacific Biosciences is either an owner or licensee of the image, and not an agent for the owner. You agree to give Pacific Biosciences a credit line as follows: "Courtesy of Pacific Biosciences of California, Inc., Menlo Park, CA, USA" and also include any other credits or acknowledgments noted by Pacific Biosciences. You must include any copyright notice originally included with the images on all copies.

IMAGES ARE PROVIDED BY Pacific Biosciences ON AN "AS-IS" BASIS. Pacific Biosciences DISCLAIMS ALL REPRESENTATIONS AND WARRANTIES, EXPRESS, IMPLIED OR STATUTORY, INCLUDING, BUT NOT LIMITED TO, NON-INFRINGEMENT, OWNERSHIP, MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL Pacific Biosciences BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, PUNITIVE, OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER WITH RESPECT TO THE IMAGES.

You agree that Pacific Biosciences may terminate your access to and use of the images located on the PacificBiosciences.com website at any time and without prior notice, if it considers you to have violated any of the terms of this Image Use Agreement. You agree to indemnify, defend and hold harmless Pacific Biosciences, its officers, directors, employees, agents, licensors, suppliers and any third party information providers to the Site from and against all losses, expenses, damages and costs, including reasonable attorneys' fees, resulting from any violation by you of the terms of this Image Use Agreement or Pacific Biosciences' termination of your access to or use of the Site. Termination will not affect Pacific Biosciences' rights or your obligations which accrued before the termination.

I have read and understand, and agree to, the Image Usage Agreement.

I disagree and would like to return to the Pacific Biosciences home page.

Pacific Biosciences
Contact:

Recent Publications Demonstrate Rapid Adoption of SMRT Sequencing for Plant and Animal Transcriptome Analysis

Monday, May 22, 2017

MENLO PARK, Calif., May 22, 2017 (GLOBE NEWSWIRE) — A series of recent peer-reviewed scientific publications highlights the growing use of Single Molecule, Real-Time (SMRT®) Sequencing to annotate plant and animal genomes. These reports from the genomics community show that PacBio sequencing consistently detects more genes, alternative splicing events, and other important elements compared with competing sequencing platforms. Characterizing these elements is essential for a deeper understanding of these organisms as well as for breeding efforts to improve crops and livestock.

Genome annotation using short-read technology (RNA-seq) is challenging because individual reads are too short to span entire gene transcripts, which can be multiple kilobases long. As a result, scientists must stitch together many short reads using error-prone assembly processes. Alternative splicing, in which the same gene expresses functionally different transcripts, is particularly difficult to detect with short reads because segments of those transcripts are often conflated during assembly. SMRT Sequencing produces significantly longer reads, achieving mean lengths of 10-18 kb, allowing scientists to generate full-length transcripts or isoforms without introducing errors from assembly algorithms. For transcriptome analysis, this is known as the Iso-Seq™ method because it produces full-length isoform sequences.

“Scientists are embracing the Iso-Seq method to produce
a more complete genetic view of everything from important crops, such as wheat and barley, to livestock, including chickens,” said Kevin Corcoran, Senior Vice President of Market Development for Pacific Biosciences. “The beauty of this approach is that SMRT Sequencing provides a lot of novel information about gene content even for organisms that lack a reference assembly or have incredibly complex genomes. With our latest instrument, the Sequel System, we anticipate that more groups will adopt SMRT Sequencing as a fast and affordable means of generating the most comprehensive transcriptomes possible.”

“In our analysis of gene activity in chickens, SMRT Sequencing allowed us to overcome challenges that had limited our view of genes in this bird for years,” said David Burt, Professor at the University of Queensland, Genomics Initiative, who serves on the steering committee for the Functional Annotation of Animal Genomes (FAANG) Project. “A major advance over previous methods was the accurate characterization of all alternative splice transcripts – from start to end – allowing us to define all transcription start sites. More importantly, for the first time we were able to define accurate long non-coding RNAs. Based on this success, we anticipate implementing the Iso-Seq method for transcriptomic studies of many livestock species across the FAANG consortium as the surest path to fully characterizing the gene content in these animals.”

Recent Iso-Seq publications include:

Normalized long read RNA sequencing in chicken reveals transcriptome complexity similar to human, BMC Genomics
Principal investigator David Burt and his team studied embryonic and brain tissue from chickens, finding 70% more genes than were previously known to exist in this animal.

An improved assembly and annotation of the allohexaploid wheat genome identifies complete families of agronomic genes and provides genomic evidence for chromosomal translocations, Genome Research

Led by Matthew Clark, this project analyzed gene content in Chinese
Spring bread wheat. PacBio data revealed more than 6,600 genes missed by other technologies, and corrected more than 8,100 gene models that were inaccurate in previous annotations.

A chromosome conformation capture ordered sequence of the barley genome, Nature

Principal investigator Nils Stein and collaborators used SMRT Sequencing and other tools to characterize the transcriptome of barley. Their analysis revealed about 40,000 high-confidence genes.

Alternative splicing profile and sex-preferential gene expression in the female and male Pacific abalone Haliotis discus hannai, Gene

Senior author Young Chang
Sohn
and team reported a detailed analysis of gene content in abalone, finding that transcriptome patterns differed substantially between males and females. More than 500 genes in females and nearly 400 genes in males had multiple isoforms, i.e., evidence of alternative splicing.

Single-cell mRNA isoform diversity in the mouse brain, BMC Genomics

Scientists Kasper Karlsson and Sten Linnarsson report unexpected alternative splicing diversity in mouse brain tissue, with many cells expressing unique isoforms.

For more information, please visit https://www.pacb.com/research-focus/plant-animal-sciences/.

About Pacific Biosciences

Pacific Biosciences of California, Inc. (NASDAQ:PACB) offers sequencing systems to help scientists resolve genetically complex problems. Based on its novel Single Molecule, Real-Time (SMRT®) Sequencing technology, Pacific Biosciences’ products enable: de novo genome assembly to finish genomes in order to more fully identify, annotate and decipher genomic structures; full-length transcript analysis to improve annotations
in reference genomes, characterize alternatively spliced isoforms in important gene families, and find novel genes; targeted sequencing to more comprehensively characterize genetic variations; and real-time kinetic information for epigenome characterization. Pacific Biosciences’ technology provides high accuracy, ultra-long reads, uniform coverage, and the ability to simultaneously detect epigenetic changes. PacBio® sequencing systems, including consumables and software, provide a simple, fast, end-to-end workflow for SMRT Sequencing. More information is available at www.pacb.com.


Forward-Looking Statements

All statements in this press release that are not historical are forward-looking statements, including, among other things, statements relating to future availability, uses, quality or performance of, or benefits of using, products or technologies, and other future events. You should not place undue reliance on forward-looking statements because they involve known and unknown risks, uncertainties, changes in circumstances and other factors that are, in some cases, beyond Pacific Biosciences’ control and could cause actual results to differ materially from the information expressed or implied by forward-looking statements made in this press release. Factors that could materially affect actual results can be found in Pacific Biosciences’ most recent filings with the Securities and Exchange Commission, including Pacific Biosciences’ most recent
reports on Forms 8-K, 10-K and 10-Q, and include those listed under the caption “Risk Factors.”

Pacific Biosciences undertakes no obligation to revise or update information in this press release to reflect events or circumstances in the future, even if new information becomes available.

Contacts



Media:

Nicole Litchfield

415.793.6468

nicole@bioscribe.com



Investors:

Trevin Rard

650.521.8450

ir@pacificbiosciences.com

Primary Logo

Source: Pacific Biosciences, Inc.

News Provided by Acquire Media