PacBio Literature

The Kinnex full-length RNA kit takes total RNA as input and outputs a sequencing-ready library that results in an 8-fold throughput increase compared to typical Iso- Seq libraries. Combined with the Iso-Seq analysis in SMRT Link software, PacBio offers cost-effective isoform sequencing that does not require orthogonal sequencing methods.

PacBio® HiFi sequencing delivers highly accurate long reads for analyzing both DNA and RNA. The unique combination of accurate base calling and long read length of HiFi enable the phasing of haplotypes, coverage of high GC regions, and bisulfite-free detection of 5-methylcytosine. Together, these features allow you to explore the full extent of human genetic variation with a more comprehensive view of the genome, transcriptome, and epigenome than ever before

Long read lengths and high accuracy are essential components to successful whole transcriptome analysis. PacBio full-length RNA sequencing demonstrates a comparative advantage against ONT.

This Application note introduces the PacBio WGS Variant Pipeline that consolidates several state-of-the- art analysis tools into a single user-friendly workflow.

This technical note describes one set of experimental conditions for shearing high molecular weight genomic DNA (gDNA) derived from human whole blood using MP Biomedicals’ FastPrep-96 instrument. These results demonstrate the FastPrep-96 as a high-throughput DNA shearing option for WGS.

PacBio® HiFi long-read amplicon sequencing has demonstrated advantages for high-throughput, high- resolution eukaryotic barcode sequencing.

HiFi reads can span most repeats, making it possible to comprehensively profile variation in repeat regions and to measure any changes in methylation. PacBio has developed the Tandem repeat genotyping tool (TRGT) and Tandem repeat visualizer (TRVZ) as bioinformatics tools to accurately profile short tandem repeats, variable number tandem repeats, and macrosatellites.

Plate-based shearing using the SPEX SamplePrep 1600 MiniG homogenizer is a high-throughput and cost-effective alternative to the standard, lower-throughput options recommended for HiFi sequencing on PacBio long-read systems

With an average of eight complete genes per HiFi read, PacBio® data provides rich functional information; nearly every read contributes to the understanding of the biological functions present in the microbial community

Accurate and flexible HiFi solutions for metagenomics and microbiome sequencing allow researchers to study microbes and microbial communities in high resolution without the need for culturing.

This technical note, including the description of the PCR assay, is intended as an example guidance for the development of an amplicon assay for use on PacBio systems, and is neither optimized nor supported by PacBio.

With PacBio Single Molecule, Real-Time (SMRT) Sequencing on the Sequel IIe System you can characterize whole genomes and transcriptomes with just one SMRT Cell. Explore our applications and pricing to get your sequencing project started.

Access many benefits of PacBio® HiFi long-read sequencing at a fraction of the cost with Twist Bioscience long-read sequencing panels. Twist Bioscience’s target enrichment technology provides high capture efficiency and exceptional uniformity across target regions.

In this Application Note, we demonstrate targeted HiFi sequencing assays combined with a new informatics method for the combined analysis of SMN1 and SMN2.

In this Application note, we focus on an amplicon- sequencing based approach for the analysis of the entire GBA gene and its pseudogene with PacBio® HiFi sequencing. We also highlight the benefits of RNA isoform sequencing (Iso-Seq® method) to generate insights into the transcriptional impact of GBAP1.