PacBio Literature

The Kinnex 16S rRNA kit increases throughput by 12-fold using the MAS- Seq concatenation technology. We show that more read depth allows for higher multiplexing capacity (up to 1,536-plex) and allows more identified species per sample. Coupled with flexible multiplexing strategies and a SMRT® Link bioinformatics workflow, users can now achieve cost-effective full-length 16S rRNA gene sequencing for species- and strain-level identification for microbiome studies.

The outstanding accuracy of HiFi long-read sequencing allows you to get the most out of each Revio run. Discover how you can access more information at reduced cost and higher throughput for each of your sequencing projects.

In this whitepaper, we discuss the features of bulk and single-cell isoform sequencing with the Iso-Seq method and their applications in human genomics.

The LightBench from Yourgene Health uses Ranger Technology to deliver accurate size selection for whole genome sequencing and includes the ability to perform fragment length analysis for additional quality control. With an automated workflow to optimize walk-away time, the LightBench offers a scalable size selection solution for the preparation of SMRTbell gDNA libraries for HiFi sequencing.

The Kinnex full-length RNA kit takes total RNA as input and outputs a sequencing-ready library that results in an 8-fold throughput increase compared to typical Iso- Seq libraries. Combined with the Iso-Seq analysis in SMRT Link software, PacBio offers cost-effective isoform sequencing that does not require orthogonal sequencing methods.

PacBio® HiFi sequencing delivers highly accurate long reads for analyzing both DNA and RNA. The unique combination of accurate base calling and long read length of HiFi enable the phasing of haplotypes, coverage of high GC regions, and bisulfite-free detection of 5-methylcytosine. Together, these features allow you to explore the full extent of human genetic variation with a more comprehensive view of the genome, transcriptome, and epigenome than ever before

Long read lengths and high accuracy are essential components to successful whole transcriptome analysis. PacBio full-length RNA sequencing demonstrates a comparative advantage against ONT.

This Application note introduces the PacBio WGS Variant Pipeline that consolidates several state-of-the- art analysis tools into a single user-friendly workflow.

This technical note describes one set of experimental conditions for shearing high molecular weight genomic DNA (gDNA) derived from human whole blood using MP Biomedicals’ FastPrep-96 instrument. These results demonstrate the FastPrep-96 as a high-throughput DNA shearing option for WGS.

PacBio® HiFi long-read amplicon sequencing has demonstrated advantages for high-throughput, high- resolution eukaryotic barcode sequencing.

HiFi reads can span most repeats, making it possible to comprehensively profile variation in repeat regions and to measure any changes in methylation. PacBio has developed the Tandem repeat genotyping tool (TRGT) and Tandem repeat visualizer (TRVZ) as bioinformatics tools to accurately profile short tandem repeats, variable number tandem repeats, and macrosatellites.

Plate-based shearing using the SPEX SamplePrep 1600 MiniG homogenizer is a high-throughput and cost-effective alternative to the standard, lower-throughput options recommended for HiFi sequencing on PacBio long-read systems

This technical note, including the description of the PCR assay, is intended as an example guidance for the development of an amplicon assay for use on PacBio systems, and is neither optimized nor supported by PacBio.

With PacBio Single Molecule, Real-Time (SMRT) Sequencing on the Sequel IIe System you can characterize whole genomes and transcriptomes with just one SMRT Cell. Explore our applications and pricing to get your sequencing project started.

Access many benefits of PacBio® HiFi long-read sequencing at a fraction of the cost with Twist Bioscience long-read sequencing panels. Twist Bioscience’s target enrichment technology provides high capture efficiency and exceptional uniformity across target regions.