PacBio Literature

With PacBio long reads you can easily and cost-effectively sequence the full length of amplicons that target genes or regions of interest, from several hundred base pairs to kilobase scale. Highly accurate HiFi reads allow you to target across types of variation, from single nucleotide variants and indels, to structural variants. It also enables unambiguous haplotype resolution through direct phasing. HiFi sequencing can reveal biology that other technologies miss, like low-complexity regions, repeat expansions, GC-rich promoter regions, and insertion sites of transposable elements.

This application note details the PureTarget carrier panel targets, presents example performance, and provides a technical overview of the methods, workflow, and analysis pipeline.

PureTarget offers an easy and scalable workflow to capture repeat expansions and brings you from sample to answer in three days. Compatible with the RevioTM and Sequel® II systems, PureTarget libraries can be multiplexed with up to 48 samples, generating deep coverage across the 20-gene target panel.

The Iso-Seq method, full-length RNA sequencing using PacBio HiFi sequencing, can sequence full- length isoforms without the need for computational inferencing, reducing errors in isoform identification. The KinnexTM RNA kits, based on the MAS-Seq method for concatenating amplicons such as cDNA, further increases throughput on the PacBio long-read sequencers. Here, we highlight publications using Iso- Seq in neuroscience research to show how bulk and single-cell Iso-Seq data help identify novel isoforms, determine disease-specific isoform expressions, delineate splicing patterns during brain development, and help identify new drug candidates.

SMRT® Link software tools for PacBio® HiFi sequencing systems are designed to set up and monitor sequencing runs, review performance metrics, and analyze, visualize, and annotate sequencing data. SMRT Link is available for both local installation and cloud access, offering flexibility to work in different environments depending on your lab’s needs.

PacBio Insight is a comprehensive remote support service that maintains optimal performance of your PacBio sequencing systems at no additional cost. With its secure remote-communication software, PacBio Insight uses information about function and performance directly from the instrument to prevent and diagnose performance issues and ensure operational continuity. Performance monitoring is governed by permission settings and is viewable through an audit trail, so you control what information is shared.

The Onso library preparation kits are used to create libraries for the PacBio Onso short-read sequencing system.

Vega system specification sheet

The Kinnex single-cell RNA kit takes as input single- cell cDNA and outputs a sequencing-ready library that results in a 16-fold throughput increase compared to regular single-cell Iso-Seq libraries.

Kinnex kits can be used as a general concatenation kit for cDNA or gDNA amplicons, which can increase the throughput by 8-, 12-, or 16-fold, and decrease sequencing cost. Read the tech note to see how you can use or modify your amplicon for cost-effective sequencing with Kinnex kits on PacBio’s long read sequencers.

PacBio long-read HiFi sequencing allows you to sequence the entire AAV genome with extraordinary accuracy and completeness so that you can quickly discover and optimize gene therapy vectors.

With highly accurate long reads (HiFi reads), powered by Single Molecule, Real-Time (SMRT) sequencing technology, you can more comprehensively validate gene editing techniques such as CRISPR-Cas9 approaches.

Highly accurate long-read sequencing provides more complete and unbiased information crucial for cell and gene therapy development, ensuring the confidence you need to advance your projects.

PacBio long-read sequencing technology and Form Bio’s streamlined data analysis now offer an all-in-one solution to optimize your AAV vector designs. This workflow can help enable desired gene therapy outcomes, providing you with the confidence and assurance to take your gene therapy research to new heights and stay ahead of the curve.

With Single Molecule, Real-Time (SMRT®) sequencing on the Sequel® IIe systems, you can sequence AAV genome populations to identify truncation, mutation, and host integration events. The AAV workflow from PacBio® accommodates both scAAV and ssAAV constructs with easy on-instrument HiFi read generation