PacBio Literature

PacBio Insight is a comprehensive remote support service that maintains optimal performance of your PacBio sequencing systems at no additional cost. With its secure remote-communication software, PacBio Insight uses information about function and performance directly from the instrument to prevent and diagnose performance issues and ensure operational continuity. Performance monitoring is governed by permission settings and is viewable through an audit trail, so you control what information is shared.

The Onso library preparation kits are used to create libraries for the PacBio Onso short-read sequencing system.

Vega system specification sheet

The Kinnex single-cell RNA kit takes as input single- cell cDNA and outputs a sequencing-ready library that results in a 16-fold throughput increase compared to regular single-cell Iso-Seq libraries.

Kinnex kits can be used as a general concatenation kit for cDNA or gDNA amplicons, which can increase the throughput by 8-, 12-, or 16-fold, and decrease sequencing cost. Read the tech note to see how you can use or modify your amplicon for cost-effective sequencing with Kinnex kits on PacBio’s long read sequencers.

PacBio long-read HiFi sequencing allows you to sequence the entire AAV genome with extraordinary accuracy and completeness so that you can quickly discover and optimize gene therapy vectors.

With highly accurate long reads (HiFi reads), powered by Single Molecule, Real-Time (SMRT) sequencing technology, you can more comprehensively validate gene editing techniques such as CRISPR-Cas9 approaches.

Highly accurate long-read sequencing provides more complete and unbiased information crucial for cell and gene therapy development, ensuring the confidence you need to advance your projects.

PacBio long-read sequencing technology and Form Bio’s streamlined data analysis now offer an all-in-one solution to optimize your AAV vector designs. This workflow can help enable desired gene therapy outcomes, providing you with the confidence and assurance to take your gene therapy research to new heights and stay ahead of the curve.

With Single Molecule, Real-Time (SMRT®) sequencing on the Sequel® IIe systems, you can sequence AAV genome populations to identify truncation, mutation, and host integration events. The AAV workflow from PacBio® accommodates both scAAV and ssAAV constructs with easy on-instrument HiFi read generation

High-quality HiFi reads detect a wide range of cancer-specific somatic variation including single nucleotide variants (SNVs), structural variants (SVs), insertions and deletions (indels), copy number variations (CNVs), and methylation in a single assay. HiFi sequencing shows improved somatic variant calling accuracy with less sequencing relative to other technologies.

Kinnex offers full-length isoform discovery and abundance information, cell type-specific isoform discovery, and species-level metagenomics community identification that are difficult to capture with short reads.

The Revio™ system empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

Highlighting publications using Iso-Seq in cancer research to demonstrate how bulk and single-cell Iso- Seq data detects fusion genes, identifies novel isoforms that might serve as neoepitopes for vaccine candidates, and traces clonal evolution.

Reveal the complexity of the human genome to power human disease research — With the advancement of highly accurate long-read sequencing, missing pieces could be resolved and fully complete the genome. PacBio HiFi sequencing gave rise to this achievement and has dramatically improved understanding of the human genome by resolving gaps that are unattainable by other sequencing methods, accurately mapping complex regions, and enabling more contiguous genome assemblies.