PacBio Literature

Highly accurate long reads — HiFi reads — are the new benchmark for full-length 16S, metagenome profiling, and metagenome assembly. Determine community composition at the species or strain level with competitively priced full-length 16S sequencing. Generate hundreds of high-quality (HQ) metagenome assembled genomes (MAGs), many of which are circular single contigs. Profile metagenomes efficiently and cost-effectively with an average of eight full-length genes in every HiFi read. Leverage epigenomic data to associate contigs and plasmids from closely related strains.

Microbial genomics with PacBio long-read HiFi sequencing is accurate, scalable, and cost effective. Automated end-to-end workflows from extraction to analysis and optimized kits drive greater throughput for HiFi microbial sequencing at lower cost including microbial whole genome sequencing, full-length 16s/ITS sequencing, viral sequencing and shotgun metagenome sequencing.

Make reference-quality genomes your new standard practice with highly accurate long reads—HiFi reads—and the PacBio end-to-end workflow. Generate closed chromosomes and plasmids. Identify ever-evolving genes associated with toxicity, virulence, and antimicrobial resistance. Precisely identify strains, serotypes, and plasmids to help track pathogen outbreaks in human, plant, and animal species, through food systems, hospitals, and communities. More comprehensively characterize microbes to facilitate scientific breakthroughs and discovery

This Application note provides the workflow for the detection of somatic small variants, structural variants, and methylation for paired tumor-normal samples with HiFi whole genome sequencing (WGS)

PacBio-developed tools offer an excellent analysis experience as they are designed specifically for HiFi data. There are multiple ways to access PacBio analyses.

This technical note describes the experimental conditions of a modified workflow preparing PacBio Kinnex single-cell libraries with Twist exome enrichment kits.

Powered by the exceptional accuracy of HiFi sequencing and the Tandem Repeat Genotyping Tool (TRGT), the PacBio® PureTarget™ repeat expansion panel offers more comprehensive genotyping for 20 of the most important repeat expansions for human health. This application note demonstrates the performance of the PureTarget repeat expansion panel, and presents the PureTarget panel as a scalable, more comprehensive solution for profiling repeat expansions, compared to legacy genotyping and next-generation sequencing methods.

This application note demonstrates that DNA extracted from whole blood and saliva samples using the Nanobind kits yields high-quality HiFi sequencing data.

In this whitepaper, we discuss the features of full- length and single-cell isoform sequencing with the Iso-Seq method and their applications in plant and animal research. We show how combining the Iso-Seq method with Kinnex kits further increases throughput, making PacBio long reads a cost-effective RNA sequencing solution.

The Kinnex single-cell RNA kit is compatible with cDNA generated using the Visium HD 3' Spatial Gene Expression assay for creating a sequencing-ready library that can run on PacBio sequencing systems.

SMRT Link Cloud is a free, PacBio-hosted version of SMRT Link that removes the need for IT maintenance.

With HiFi target enrichment you can easily and cost-effectively sequence just the genes you care about at scale. Design custom gene panels with our partners at Twist, sequence them with long and accurate HiFi reads, and get all the benefits you expect from HiFi reads: comprehensive detection of single nucleotide variants, structural variants, and indels. HiFi target enrichment is the only technology that can resolve complex gene families like HLA or Cytochrome P450 genes with haplotype resolution.​

Other sequencing methods suffer from shortcomings like an inability to capture the backbone sequence or low accuracy in difficult genomic regions such as homopolymers or regions of high GC content. PacBio® HiFi sequencing overcomes these limitations and allows you to capture the full plasmid sequence, including backbone and difficult regions.

The whole genome sequencing (WGS) application requires an up-front mechanical DNA shearing step to transform genomic DNA into fragment sizes appropriate for HiFi sequencing. With the Hamilton Microlab Prep system, this step can be performed in as little as 22 minutes for 24 samples at the cost of a pipette tip per sample. Here we describe the required equipment and consumables, best practices, and procedure for shearing DNA on the Microlab Prep system.

Fiber-seq is a powerful new long-read whole genome sequencing assay that overcomes the challenges imposed by short-read chromatin sequencing.