PacBio Literature

SMRT Link Cloud is a free, PacBio-hosted version of SMRT Link that removes the need for IT maintenance.

With HiFi target enrichment you can easily and cost-effectively sequence just the genes you care about at scale. Design custom gene panels with our partners at Twist, sequence them with long and accurate HiFi reads, and get all the benefits you expect from HiFi reads: comprehensive detection of single nucleotide variants, structural variants, and indels. HiFi target enrichment is the only technology that can resolve complex gene families like HLA or Cytochrome P450 genes with haplotype resolution.​

Other sequencing methods suffer from shortcomings like an inability to capture the backbone sequence or low accuracy in difficult genomic regions such as homopolymers or regions of high GC content. PacBio® HiFi sequencing overcomes these limitations and allows you to capture the full plasmid sequence, including backbone and difficult regions.

The whole genome sequencing (WGS) application requires an up-front mechanical DNA shearing step to transform genomic DNA into fragment sizes appropriate for HiFi sequencing. With the Hamilton Microlab Prep system, this step can be performed in as little as 22 minutes for 24 samples at the cost of a pipette tip per sample. Here we describe the required equipment and consumables, best practices, and procedure for shearing DNA on the Microlab Prep system.

Fiber-seq is a powerful new long-read whole genome sequencing assay that overcomes the challenges imposed by short-read chromatin sequencing.

With PacBio long reads you can easily and cost-effectively sequence the full length of amplicons that target genes or regions of interest, from several hundred base pairs to kilobase scale. Highly accurate HiFi reads allow you to target across types of variation, from single nucleotide variants and indels, to structural variants. It also enables unambiguous haplotype resolution through direct phasing. HiFi sequencing can reveal biology that other technologies miss, like low-complexity regions, repeat expansions, GC-rich promoter regions, and insertion sites of transposable elements.

HiFi systems brochure

Application brief — What can you do with one SMRT Cell

Vega benchtop system brochure

See it all with HiFi sequencing on the Revio™ system

This application note details the PureTarget carrier panel targets, presents example performance, and provides a technical overview of the methods, workflow, and analysis pipeline.

PureTarget offers an easy and scalable workflow to capture repeat expansions and brings you from sample to answer in three days. Compatible with the RevioTM and Sequel® II systems, PureTarget libraries can be multiplexed with up to 48 samples, generating deep coverage across the 20-gene target panel.

The Iso-Seq method, full-length RNA sequencing using PacBio HiFi sequencing, can sequence full- length isoforms without the need for computational inferencing, reducing errors in isoform identification. The KinnexTM RNA kits, based on the MAS-Seq method for concatenating amplicons such as cDNA, further increases throughput on the PacBio long-read sequencers. Here, we highlight publications using Iso- Seq in neuroscience research to show how bulk and single-cell Iso-Seq data help identify novel isoforms, determine disease-specific isoform expressions, delineate splicing patterns during brain development, and help identify new drug candidates.

SMRT® Link software tools for PacBio® HiFi sequencing systems are designed to set up and monitor sequencing runs, review performance metrics, and analyze, visualize, and annotate sequencing data. SMRT Link is available for both local installation and cloud access, offering flexibility to work in different environments depending on your lab’s needs.

PacBio Insight is a comprehensive remote support service that maintains optimal performance of your PacBio sequencing systems at no additional cost. With its secure remote-communication software, PacBio Insight uses information about function and performance directly from the instrument to prevent and diagnose performance issues and ensure operational continuity. Performance monitoring is governed by permission settings and is viewable through an audit trail, so you control what information is shared.