HiFi difference
From eye-opening tech comparisons to inspiring examples of PacBio HiFi technology in action — this blog series explores what sets HiFi sequencing apart.
Featured article
THE HIFI DIFFERENCE – A BETTER CELL ATLAS WITH FULL-LENGTH ISOFORM SEQUENCING
Understanding the myriad of different cell types, states, and their functions is essential to understanding biology. Cell atlas projects and consortia aim to map different cell types in tissues, organs,…
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The HiFi difference: powering biodiversity genomics
In a new preprint, entitled Realizing the promise of biodiversity genomics with highly accurate long reads, a group of researchers from Utah State, BYU, the LOEWE Centre for Translational Biodiversity…
The HiFi difference: resolving the most complex genomic regions
The combined accuracy, read length, and coverage uniformity of PacBio HiFi sequence reads enables the characterization of many previously inaccessible regions in the genome. Two new preprints by Oscar Rodriguez,…
The HiFi difference: enabling the human pangenome reference
A few weeks ago, we described the use of HiFi sequencing to drive the paradigm shift from a single reference genome to a pangenome derived from a diverse set of…
The HiFi difference – More precise genomes for precision medicine
More precise data provided by PacBio HiFi sequencing can have a marked impact on the resolving power in genetic association studies and decrease the required cohort sizes. As a recent…
The HiFi difference — Not all gigabases are created equal
PacBio HiFi sequencing for the de novo genome assembly of microbial, fungal, plant, animal and human genomes has been described in hundreds of publications, and its best-in-class performance has been…
The HiFi difference – Getting the right answer
The combined accuracy and length of PacBio HiFi reads is utilized by an ever-increasing number of researchers, with numerous new publications and preprints appearing every week. Several months ago, Illumina…
The HiFi difference – haplotype phasing in genome assembly
Most higher organisms are diploid, meaning that each cell carries in its nucleus two copies of the individual’s genome, one from its mother and one from its father. Accurately separating…
The HiFi difference – sequencing telomeres
The scientific community is still abuzz from last week’s announcement of the amazing achievement of the first complete sequence of a human genome by the Telomere-to-Telomere (T2T) Consortium. Assembling genomes…
The HiFi difference – full-length RNA sequencing
Through the development of long-read, full-length RNA sequencing methods, the scientific community has recognized a fundamental paradigm shift, “from ‘gene-centric’ to ‘isoform-centric’ understanding of human transcriptomes, owing to the frequent…