Blog
This blog features voices from PacBio — and our partners and colleagues — discussing the latest research, publications, and updates about HiFi sequencing.
Blog
SMRT Sequencing Enables Discovery of Epigenetic Driver of C. diff Persistence
How do pernicious pathogens like Clostridioides difficile spread through hospitals and persist so tenaciously in the human gut, leading to about half a million infections and 30,000 deaths each year? …
Long-Read Sequencing Could Improve the Sensitivity and Precision of 16S Studies Says Jackson Lab Study
It’s time to revisit the way scientists are using 16S rRNA gene sequencing to study microorganisms, according to a team of Jackson Laboratory researchers. Popular targets for taxonomy and phylogeny…
Two Review Articles Assess Structural Variation in Human Genomes
Two recent review articles discuss the idea that structural variants (SVs) — genetic differences that involve at least 50 base pairs — are numerous, important to human biology, and best…
Award-Winning Sequel II System Sets a New Standard for Long-Read Sequencing
UPDATE: We’re proud to have released the next evolution of the Sequel System: The Sequel IIe. Launched in October 2020, the new system features advanced on-instrument data processing and cloud…
Users Report on SMRT Sequencing: Sequel II System, HiFi Reads, Iso-Seq Analysis, Microbiomes, and More
We’d like to extend a sincere thanks to everyone who attended our two-day North America User Group Meeting, held this year at our Certified Service Provider, the University of Delaware…
SMRT Sequencing to Help Reveal Secrets of the Soil in Understudied South-East Asian Rainforests
The most important creatures in a tropical rainforest aren’t necessarily the ones you can see. They work their magic underground, recycling organic matter and processing and transporting vital nutrients for…
Webinar Summary: Need accurate isoform-level characterization? Iso-Seq is the answer
Traditional RNA-Seq is done by fragmenting cDNA, and then sequencing the fragmented reads with paired-end sequencing. The problem comes when trying to identify the full-length isoform during assembly. This is…
At ASHG Workshop, Customers Describe Long-Read Sequencing of Human Genomes for Disease Gene Discovery and Population Studies
We were delighted to host an educational workshop at last month’s annual meeting of the American Society of Human Genetics (ASHG), where we had the opportunity to feature talks from…
Mapping the NLRome: Research Teams Turn to SMRT Sequencing to Trace Plant Immunity
There’s the genome, the transcriptome, the microbiome… and now the NLRome? Breeders and pathologists have long been interested in uncovering the secrets of plant immunity, and much of their attention…
Double Mutations in Oncogene May Improve Patient Response to Targeted Therapy
The PIK3CA oncogene has been the target of intense research scrutiny for decades. Remarkably, though, a new paper in Science today reports completely novel findings about compound mutations that are…
ASHG 2019: CoLab Session Highlights Structural Variation and Transcriptome Sequencing
At the annual meeting of the American Society of Human Genetics in Houston, PacBio scientists presented how our Sequel II System performs for structural variant (SV) detection and for whole…
Adaptive Selection of CNVs: UW Team Applies SMRT Sequencing to the Melanesian Genome
In a new Science publication, researchers from the University of Washington and other institutions report detailed analyses revealing the adaptive importance of copy number variants (CNVs) acquired from Denisovan and…
In New Tibetan Genome Assembly, Variants for Living at Altitude and the Imprint of Archaic DNA
A recent bioRxiv preprint reports efforts to sequence the genome of a Tibetan individual and detect the genetic underpinning of adaptive traits associated with tolerating high altitude. The authors used…
Review: Long-Read Sequencing Helps Uncover Genetic Basis for Rare Disease
A new review article nicely sums up the utility of long-read sequencing for solving rare diseases that cannot be explained by other methods. The paper, published in the Journal of…
Keeping a Close Eye on MRSA: Lessons Learned from PacBio Sequencing Surveillance
When MRSA hits your hospital, what do you do? If you’re located in Europe or other places where infection rates are still relatively low, you can take a seek-and-destroy approach,…