PacBio Literature

Understanding the complexity of cancer genomes requires methods that can reveal the full breadth of cancer genomic variation, from SNVs and indels to structural variants (SVs), copy number variants (CNVs), and differential methylation. HiFi long-read sequencing allows cancer researchers to characterize the complete spectrum of somatic and germline variation, including SVs and methylation, yielding a clearer view of cancer biology and helping to identify new therapeutic targets and inform clinical decision making.

Comprehensive genome sequencing data integrated with chromatin interaction information is crucial for complete, chromosome-scale genome assembly. CiFi (pronounced “Sci-Fi”) is a new application developed by the sequencing community that integrates chromatin conformation capture (3C) with PacBio HiFi long-read sequencing to generate highly accurate, multi-contact reads.

Highly accurate long reads — HiFi reads — are the new benchmark for full-length 16S, metagenome profiling, and metagenome assembly. Determine community composition at the species or strain level with competitively priced full-length 16S sequencing. Generate hundreds of high-quality (HQ) metagenome assembled genomes (MAGs), many of which are circular single contigs. Profile metagenomes efficiently and cost-effectively with an average of eight full-length genes in every HiFi read. Leverage epigenomic data to associate contigs and plasmids from closely related strains.

Microbial genomics with PacBio long-read HiFi sequencing is accurate, scalable, and cost effective. Automated end-to-end workflows from extraction to analysis and optimized kits drive greater throughput for HiFi microbial sequencing at lower cost including microbial whole genome sequencing, full-length 16s/ITS sequencing, viral sequencing and shotgun metagenome sequencing.

Make reference-quality genomes your new standard practice with highly accurate long reads—HiFi reads—and the PacBio end-to-end workflow. Generate closed chromosomes and plasmids. Identify ever-evolving genes associated with toxicity, virulence, and antimicrobial resistance. Precisely identify strains, serotypes, and plasmids to help track pathogen outbreaks in human, plant, and animal species, through food systems, hospitals, and communities. More comprehensively characterize microbes to facilitate scientific breakthroughs and discovery

This Application note provides the workflow for the detection of somatic small variants, structural variants, and methylation for paired tumor-normal samples with HiFi whole genome sequencing (WGS)

PacBio-developed tools offer an excellent analysis experience as they are designed specifically for HiFi data. There are multiple ways to access PacBio analyses.

This technical note describes the experimental conditions of a modified workflow preparing PacBio Kinnex single-cell libraries with Twist exome enrichment kits.

Powered by the exceptional accuracy of HiFi sequencing and the Tandem Repeat Genotyping Tool (TRGT), the PacBio® PureTarget™ repeat expansion panel offers more comprehensive genotyping for 20 of the most important repeat expansions for human health. This application note demonstrates the performance of the PureTarget repeat expansion panel, and presents the PureTarget panel as a scalable, more comprehensive solution for profiling repeat expansions, compared to legacy genotyping and next-generation sequencing methods.

Unravel the complexity of plant and animal genomes with HiFi sequencing to harness agrigenomics insights for breeding, crop protection, and genome engineering.

This application note demonstrates that DNA extracted from whole blood and saliva samples using the Nanobind kits yields high-quality HiFi sequencing data.

Unravel the complexity of plant and animal biology for any species with HiFi sequencing. Discover more about species and populations with more accurate genomes, transcriptomes, and epigenomes.

PacBio® provides cutting-edge sequencing that allows scientists to unlock the genetic diversity of all species on earth. These tools can fuel discoveries in agriculture, biodiversity, and environmental health, and build toward a sustainable future.

In this whitepaper, we discuss the features of full- length and single-cell isoform sequencing with the Iso-Seq method and their applications in plant and animal research. We show how combining the Iso-Seq method with Kinnex kits further increases throughput, making PacBio long reads a cost-effective RNA sequencing solution.

The Kinnex single-cell RNA kit is compatible with cDNA generated using the Visium HD 3' Spatial Gene Expression assay for creating a sequencing-ready library that can run on PacBio sequencing systems.