Genome assembly of a tropical maize inbred line provides insights into structural variation and crop improvement.

Authors: Yang, Ning and Liu, Jie and Gao, Qiang and Gui, Songtao and Chen, Lu and Yang, Linfeng and Huang, Juan and Deng, Tianquan and Luo, Jingyun and He, Lijuan and Wang, Yuebin and Xu, Pengwei and Peng, Yong and Shi, Zhuoxing and Lan, Liu and Ma, Zhiyun and Yang, Xin and Zhang, Qianqian and Bai, Mingzhou and Li, San and Li, Wenqiang and Liu, Lei and Jackson, David and Yan, Jianbing

Maize is one of the most important crops globally, and it shows remarkable genetic diversity. Knowledge of this diversity could help in crop improvement; however, gold-standard genomes have been elucidated only for modern temperate varieties. Here, we present a high-quality reference genome (contig N50 of 15.78?megabases) of the maize small-kernel inbred line, which is derived from a tropical landrace. Using haplotype maps derived from B73, Mo17 and SK, we identified 80,614 polymorphic structural variants across 521 diverse lines. Approximately 22% of these variants could not be detected by traditional single-nucleotide-polymorphism-based approaches, and some of them could affect gene expression and trait performance. To illustrate the utility of the diverse SK line, we used it to perform map-based cloning of a major effect quantitative trait locus controlling kernel weight-a key trait selected during maize improvement. The underlying candidate gene ZmBARELY ANY MERISTEM1d provides a target for increasing crop yields.

Journal: Nature genetics
DOI: 10.1038/s41588-019-0427-6
Year: 2019

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