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Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems.

Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing

Description

This procedure describes the workflow for constructing SMRTbell libraries with PCR amplification from as little as 1 ng of starting genomic DNA using the Ampli-Fi protocol. Sequencing libraries prepared with this procedure are compatible with PacBio Revio and Vega systems.

Procedure & checklist — Generating PureTarget repeat expansion panel libraries

Description

This procedure describes the workflow for generating PureTarget repeat expansion libraries.

Procedure & checklist — Preparing Iso-Seq libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing Iso-Seq libraries using SMRTbell prep kit 3.0 from RNA for sequencing on PacBio HiFi systems

Procedure & checklist — Preparing Kinnex libraries from 16S rRNA amplicons

Description

This procedure provides instructions for generating Kinnex libraries from full-length 16S amplicons for sequencing on PacBio HiFi systems.

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio HiFi systems.

Procedure & checklist — Preparing Kinnex libraries using the Kinnex full-length RNA kit

Description

This procedure describes the workflow for constructing Kinnex full-length RNA libraries from total RNA samples for sequencing on PacBio HiFi systems.

Procedure & checklist — Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems. Amplicons may be indexed during PCR, or during library preparation with SMRTbell indexed adapters.

Procedure & checklist — Preparing multiplexed whole genome and amplicon libraries using the HiFi plex prep kit 96

Description

This procedure describes the workflow for constructing multiplexed whole genome sequencing (WGS) and amplicon libraries using the HiFi plex prep kit 96 for sequencing on PacBio HiFi systems. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.

Procedure & checklist — Preparing whole genome libraries using the HiFi prep kit 96

Description

This procedure describes the high throughput workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the Hamilton NIMBUS Presto system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the Hamilton NIMBUS Presto system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of human whole blood using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for cultured bacteria on the Hamilton NIMBUS Presto system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from cultured Gram-negative and Gram-positive bacteria using the Hamilton NIMBUS Presto robotic system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on Hamilton NIMBUS Presto system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for non-human mammalian blood NHMB on the Hamilton NIMBUS Presto system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50-300 kb) DNA from 200 μL of non-human mammalian blood (NHMB) using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.