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July 22, 2025  |  Infectious disease research

Development and validation of HIV SMRTcap for the characterization of HIV-1 reservoirs across tissues and subtypes

Authors: Ghazal Sadri, Steven T. Nadakal, William Lauer, Justin Kos, Parmit K. Singh, Erin M. Elliott, Catherine W. Kaiser, Easton E. Ford, Nadia Richardson, Elizabeth Hudson, Noemi L. Linden, Ali Danesh, James Powell, Peter Warburton, Juan Soto, Matthew Emery, Gintaras Deikus, Guinevere Q. Lee, Susanna L. Lamers, Steven J. Reynolds, Ronald Galiwango, Jessica L. Prodger, Stephen Tomusange, Taddeo Kityamuweesi, Tina Han, R. Brad Jones, Aaron A. R. Tobian, Alan N. Engelman, Robert Sebra, Susan Morgello, Andrew D Redd, David Sachs, Eric Rouchka, Melissa L. Smith

In this preprint, researchers from the University of Louisville and 20 collaborating institutions introduce a new HIV-1 reservoir analysis method using HiFi sequencing. They describe it as “a highly accurate, single-molecule HIV-1 reservoir sequencing and characterization pipeline” that enables “direct and simultaneous resolution of integration site and proviral integrity in an HIV-1 subtype-agnostic manner … with high accuracy and lower costs than comparable methods.”

Journal: BioRxiv
DOI: 10.1101/2025.05.01.651657
Year: 2025

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