Explore PacBio’s educational videos

Discover the Vega System from PacBio—delivering trusted HiFi long-read sequencing to labs of all sizes. Accessible and affordable, Vega gives you the control, read length, and accuracy you need to explore with confidence.

The Revio system adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy and direct methylation detection.

The Onso system is an innovative benchtop short-read DNA sequencing platform with an unprecedented level of accuracy using PacBio sequencing by binding (SBB) technology. Ultra-high Q40+ data quality allows you to break through limits of detection.

Go beyond the standard reference to see a more complete picture. This film explores how leading researchers are using HiFi long-read sequencing to resolve complex genomic regions and drive progress in clinical research. From supporting research on stem cell transplant matching with high-resolution Human Leukocyte Antigen (HLA) typing to advancing pharmacogenomics studies, PacBio technology provides the accuracy and read length needed to analyze challenging genes. This enables a multiomic approach for a more comprehensive understanding of biology and disease.

Explore targeted HiFi sequencing approaches with PacBio, offering a focused and cost-effective alternative to whole genome analysis for specific genes or regions. These methods enhance scalability and affordability, whether using the benchtop Vega system or the higher-throughput Revio system, providing robust coverage of difficult-to-sequence areas.

This video demonstrates how the PureTarget workflow delivers the scalability, accuracy, and efficiency required for large-scale repeat expansion analysis. Discover how its ability to consolidate testing for over a dozen genes into a single assay is enabling major diagnostic labs to streamline complex screening for conditions like ataxia. We present benchmarking data from well-characterized reference samples, showing high concordance and consistency across the Revio, Vega, and Sequel IIe systems.

PacBio Field Application Scientist Samantha Kalla details PureTarget, a powerful PCR-free method for targeted sequencing that preserves native methylation data. Learn how this CRISPR-Cas9-based approach enables the comprehensive analysis of challenging genomic regions, including GC-rich areas and repeat expansions, by enriching for targets directly from high-molecular-weight DNA.

Explore how long-read sequencing with the PacBio PureTarget repeat expansion panel is transforming the way researchers approach neurological disease genomics. In this webinar, you’ll learn how HiFi sequencing enables a comprehensive view of the human genome, helping labs modernize and simplify workflows for repeat expansion genotyping.

PacBio long-read sequencing can sequence full-length transcripts, but the capture of ultra-long transcripts (greater than 15kb) might be limited by typical cDNA synthesis methods. Nick Zomer at Radboud University Medical Center shows how using Samplix Xdrop technology to indirectly capture two genes associated with Usher syndrome type 2A, a genetic disease that results in hearing and vision loss.

As the first video in our series on PureTarget, this presentation explains the core principles of PacBio HiFi sequencing—the essential foundation for applications such as highly accurate targeted sequencing. Discover how HiFi reads provide exceptional length (typically 15–25 kb, up to 100 times longer than short reads) and Q33 (99.9%) accuracy, which are crucial for comprehensive genomic insights from native DNA.

In this webinar, discover how long-read single-cell RNA sequencing with the PacBio Kinnex single-cell RNA kit successfully validated shared mis-splicing patterns in adult and pediatric AML. See how innovative computational approaches helped identify dominant splicing subtypes that drive disease progression, providing insight into underlying mechanisms and revealing isoform-level differences linked to disease prognosis.

See how researchers at Radboud use Samplix Xdrop technology to capture ultra-long (18.9kb) transcripts

In this webinar, learn about the sequencing technology that is pushing neuroscience research forward, particularly in Parkinson’s disease. Discover how HiFi whole genome sequencing (WGS) and the PacBio Iso-Seq method provide complete visibility into all genomic variants, reveal crucial transcript isoform information, and ultimately enable the potential development of novel therapeutics.

Kendall Lee, PhD, highlights her work utilizing HiFi long-read low-pass sequencing for trait mapping and the potential for this tool in plant breeding. The team of researchers from HudsonAlpha and the University of Georgia compare the performance of HiFi sequencing against Illumina short reads for low-pass trait mapping of the peanut (Arachis hypogaea) genome. With its highly complex tetraploid genome, the peanut serves as an ideal test case for low-pass HiFi sequencing in trait mapping.

Explore the transformative power of PacBio HiFi sequencing for advancing research across the plant and animal kingdom. Whether you're conserving endangered species, enhancing sustainable agriculture, or protecting our environmental health, this workshop dives into how high-accuracy sequencing is being leveraged to address critical global challenges while shaping a more sustainable and brighter future.