Blog
This blog features voices from PacBio — and our partners and colleagues — discussing the latest research, publications, and updates about HiFi sequencing.
Blog
HiFi Reads Add Unparalleled Accuracy to the Long-Read Sequencing Arsenal
To enable better understanding of biology, sequencing data must be accurate and complete. This is especially true when seeking out variants and determining their implications. Luckily, technical and software improvements…
RNA Sequencing SMRT Grant Winner to Help Hone New Therapeutic Strategy in Acute Myeloid Leukemia
Variety is the spice of life, and one of the drivers of genetic variation is gene splicing. After a gene is transcribed, there are alternatively spliced transcripts that add even…
Sanger Scientists Test Sequel II System for Tree-of-Life Projects
Today we offer the final post in our blog miniseries about early access users’ experiences with the new Sequel II System. Shane McCarthy, a scientist at the University of Cambridge…
No-Amp Targeted Sequencing Used to Interrogate Disease-Associated Repeat Expansion
A new publication released in PLOS One from scientists at the Mayo Clinic offers a great look at our CRISPR/Cas9-based, amplification-free targeted sequencing method and its utility for accurately sizing…
Setting a New Gold Standard in Strain Sequencing: Webinar Describes Cannabis Pan-Genome Project
What makes one strain of cannabis have potent psychoactive properties, and another more suitable for medicinal purposes? Scientists are several steps closer to figuring it all out, thanks to PacBio…
Application Updates: Introducing Iso-Seq Express for Faster RNA Sequencing
Seeking to sequence and characterize entire transcriptomes in one go? Our new Iso-Seq protocol and reverse-transcriptase PCR kit makes it easier, speedier and cheaper. Run on the new Sequel II…
At Maryland Genomics, Scientists Deploy Sequel II System for a Wide Range of Applications
In this blog miniseries, we’re recapping presentations from early access users of the Sequel II System. Today, we summarize Luke Tallon’s report from Maryland Genomics, a PacBio Certified Service Provider….
Broad Institute Scientists Use Sequel II System for Trios, Structural Variant Detection
As we geared up for the launch of our new Sequel II System, we had the good fortune of working closely with several expert customers in an early access program….
The SMRT Special: Journal Focuses on Advances in SMRT Sequencing
Single Molecule, Real-Time (SMRT) Sequencing continues to get smarter and more powerful, with the recent launch of the Sequel II system increasing capabilities and efficiencies of the long-read DNA and…
Genome in a Bottle Consortium Describes Human Genome Structural Variation Benchmark
UPDATE: The paper has been published in Nature Biotechnology A preprint released this week from the Genome in a Bottle (GIAB) Consortium describes a benchmark set of structural variants (SVs),…
Catching up with Carola and the ‘Solar-Powered’ Sea Slug
Two years ago, Carola Greve and colleagues at the Zoological Research Museum Alexander Koenig in Bonn, Germany, were seeking to #SeqtheSlug as part of the 2017 Plant and Animal SMRT…
Unraveling Malaria Mysteries with Long-Read Sequencing
Malaria is a complicated killer, and efforts to develop effective vaccines have been hindered by gaps in our understanding of both the parasite that causes the infection, Plasmodium falciparum, and…
Review: How Long-Read Sequencing Could Make a Difference in Medical Genetics
A recent review article published in Frontiers in Genetics offers a great look at the landscape of long-read sequencing. Authors Tuomo Mantere, Simone Kersten, and Alexander Hoischen from Radboud University…
Applications and benefits of single-molecule transcriptome sequencing
When looking to understand the functional implications of genetic variability, scientists should seek out the Iso-Seq method, according to Cold Spring Harbor researchers. In a recent paper published in Frontiers…
Top 10 Tools and Tips from the SMRT Leiden Informatics Developers Meeting
Hundreds of SMRT scientists came together recently in Leiden to learn about the latest updates to PacBio technology and to showcase their data analysis tools. Extremely useful information was shared,…