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Obtain a more comprehensive set of transcript isoforms

The PacBio Isoform Sequence (Iso-Seq) method generates full-length cDNA sequences – from 5’ to the poly-A tail – to confidently characterize the full transcriptome.

Capture full-length transcripts with no assembly required

The Iso-Seq method employs high-fidelity (>99% accurate) long reads to survey transcript diversity, either broadly or in a targeted fashion, revealing key information about the frequency and types of alternative transcription to improve genome annotation and gene discovery.

Single Molecule, Real-Time (SMRT) Sequencing enhances RNA sequencing, allowing you to:

  • Discover novel genes and isoforms, even in well-characterized samples
  • Detect alternative polyadenylation motifs
  • Identify promoters and splice sites in comprehensive gene models
  • Improve accuracy of RNA-seq quantification with isoform-level resolution

Workflow: from RNA to full-length transcripts

 

Sample & Library Preparation
Prepare full-length transcripts with flexible sample and library workflows

Learn More


Sequencing
Simplify sample prep workflow and reduce project costs by using the Sequel System.

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Data Analysis
Output high-quality, full-length transcript sequences with no assembly required.

Learn More


 

Application Brief: Learn more about these best practices for long read RNA sequencing

 

 


 

New to the Iso-Seq Method?

Learn how it can help with your plant or animal project by checking out Introduction to the Iso-Seq Method: State of the Art for Full-Length Transcriptome Sequencing

 

 

 

 


 

Iso-Seq Project Calculator

 

Tell us about your plant or animal RNA sequencing project and receive an estimate on the time and materials needed using the Iso-Seq method on the Sequel System.


Spotlight: High-resolution view of transcriptional landscapes in maize and sorghum

Characterization of maize and sorghum isoforms from Iso-Seq analysis detected >40% of isoforms as novel and many alternative splicing patterns. Figure A shows the classification of isoforms of both maize and sorghum. Figure B shows the distribution of splicing patterns across tissues in both maize and sorghum.

Wang, B. et al. (2018) A comparative transcriptional landscape of maize and sorghum obtained by single-molecule sequencing. GenomeResearch 28:1-12.

To learn more about how to profile the complexity of the transcriptome with the Iso-Seq method, contact us.

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