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Sequencing Data You Can Trust

A New Paradigm in Sequencing with HiFi Reads

Advanced scientific discoveries require sequencing data that is both accurate and complete. SMRT Sequencing allows you to optimize your results with two sequencing modes so you no longer have to compromise read length for accuracy.

The benefits of highly accurate long reads (HiFi Reads)

PacBio is the only sequencing technology to offer highly accurate long reads (HiFi reads) that provide Sanger-quality accuracy (>99%) with the read lengths needed for assembly of complex genomes.


 

What are HiFi Reads?

HiFi Reads

Generate Highly Accurate Long Reads
Produce HiFi reads using the circular consensus sequencing (CCS) mode to provide base-level resolution with >99% single-molecule read accuracy for the detection of all variant types from single nucleotide to structural variants. Learn more about the advantages of long reads with high accuracy.

Produce HiFi reads using the circular consensus sequencing (CCS)

 

Explore the Difference HiFi Reads Make:


How to Achieve the Longest Reads?

Long Reads

Optimize Your Run for Even Longer Reads
Sequence read lengths in the tens of kilobases using the continuous long read (CLR) sequencing mode to enable high-quality assembly of even the most complex genomes. With SMRT Sequencing you can expect half the data in reads >50 kb and the longest reads up to 175 kb. Learn more about the advantages of long reads.

 

Generate long reads with the continuous long read (CLR) sequencing mode

 


 

Where Can You Get Started with HiFi Reads?

 

Learn more about the Sequel II System

Contact a PacBio Certified Service Provider

 

 

 

 

 


Have Questions about SMRT Sequencing?

 

Connect with a PacBio Scientist:

Selected Resources

Event

The G10K-VGP/EBP Meeting

August 27, 2019-August 30, 2019

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