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HiFi Sequencing – Unlock Your Next Great Discovery

With HiFi Sequencing you get the benefits of short reads and traditional long reads in one easy-to-use technology. Watch this short video to learn how HiFi Sequencing is empowering scientists to strive for new breakthroughs.

Explore A New Paradigm in Sequencing with HiFi Reads

Advanced scientific discoveries require sequencing data that is both accurate and complete. Single Molecule, Real-Time (SMRT) Sequencing technology has evolved to a different type of long read, known as highly accurate long reads, or HiFi reads.

HiFi Reads provide long reads with high accuracy

PacBio is the only sequencing technology to offer HiFi reads that provide accuracy of >99.9%, on par with short reads and Sanger sequencing. With HiFi reads you no longer have to compromise long read lengths for high accuracy sequencing to address your toughest biological questions.

What are HiFi Reads?

HiFi reads are a type of data produced using the circular consensus sequencing (CCS) mode on one of the PacBio Sequel Systems. HiFi reads provide base-level resolution with >99.9% single-molecule read accuracy.

HiFi Reads

HiFi reads can be used across a wide range of SMRT Sequencing applications, from whole genome sequencing for de novo assembly, comprehensive variant detection, RNA sequencing and more.

How are HiFi Reads Generated?

How are HiFi Reads Different from Traditional PacBio Long Reads?

HiFi reads are produced by calling consensus from subreads generated by multiple passes of the enzyme around a circularized template. This results in a HiFi read that is both long and accurate. In contrast, traditional PacBio long reads are generated by a single pass of the enzyme around a circularized template, which although allows for overall longer read lengths, does not provide the high accuracy characteristic of HiFi reads. Learn more about how SMRT Sequencing works.

How Does HiFi Sequencing Compare with Other Technologies?

HiFi reads let you accurately detect all types of variants, from single nucleotide to structural variants, with high precision and recall and phase haplotypes, even in hard-to-sequence regions of the genome. Explore the resources below for more information on how HiFi reads perform relative to other technologies.

Original Publication: Wenger, A. M., et al. (2019) Accurate Circular Consensus Long-read Sequencing Improves Variant Detection and Assembly of a Human Genome. Nature Biotechnology, 37, 1155–1162.

The Telomere-to-Telomere (T2T) Consortium selected HiFi sequencing to generate the first fully complete human genome assembly, 20 years after the original human genome project.

Read the Blog Post to Learn More

HiFi reads make understanding the accuracy of your sequencing data easy and straight forward, but there is a lot to know about accuracy when considering a sequencing technology.

Read the Blog Post to Learn More

In the precisionFDA Truth Challenge V2, which evaluated methods for variant calling in human genomes, approaches that use HiFi reads delivered the highest precision and recall in all categories.

Read the Blog Post to Learn More or Watch a Short Video to Understand Results from the precisionFDA Truth Challenge

What are the Benefits of HiFi Sequencing?

There are many advantage to using HiFi sequencing beyond the long read lengths and high accuracy. These include easy library preparation, low coverage requirements, small file sizes, and the fastest time to assembly. Explore the resources below better understand these advantages.

Learn more about the benefits of HiFi reads and see how they provide a single technology solution across a range of applications

Hear from PacBio users on how they are employing HiFi reads to explore genomes, transcriptomes, and metagenomes and the benefits HiFi reads provide for addressing their critical life science questions.

Discover the Many Ways Scientists are Using HiFi Reads in Their Research:

Find many more examples or try working with HiFi read data yourself in the SMRT Resources library.

How to Get Started with HiFi Sequencing?

Explore the range of SMRT Sequencing applications you can use with HiFi reads

The Sequel II System won the 2019 Scientist Top 10 Innovations Award and LSIA Award for its ability to generate longer reads with greater accuracy and throughput, at a significantly lower cost.

Learn more about HiFi sequencing using the award-winning Sequel  Systems

Connect with a PacBio scientist to have your questions answered or for a free project consultation

Contact a PacBio Certified Service Provider to run a sequencing project from start to finish

Selected Resources