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Explore PacBio’s video gallery

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Webinar: Increasing solve rates for rare and Mendelian diseases with long-read sequencing

Sund, Kristen and Wenger, Aaron
Cincinnati Children's Hospital Medical Center, PacBio

2020

Description +

Dr. Wenger gives attendees an update on PacBio’s long-read sequencing and variant detection capabilities on the Sequel II System and shares recommendations on how to design your own study using HiFi reads. Then, Dr. Sund from Cincinnati Children’s Hospital Medical Center describes how she has used long-read sequencing to solve rare neurological diseases involving complex structural rearrangements that were previously unsolved with standard methods.

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Webinar: Understanding SARS-CoV-2 and host immune response to COVID-19 with PacBio sequencing

Ashby, Meredith and Laird-Smith, Melissa and Watson, Corey
PacBio; Mt. Sinai; University of Louisville School of Medicine

2020

Description +

Studying microbial genomics and infectious disease? Learn how the PacBio Sequel II System can help advance your research, with first-hand perspectives from scientists who are investigating SARS-CoV-2 and COVID-19. In this webinar, Melissa Laird-Smith (Mt. Sinai School of Medicine) discusses her work evaluating the impact of host immune restriction in health and disease with high resolution HLA typing. She is joined by Corey Watson (University of Louisville School of Medicine) who talks about overcoming complexity to elucidate the role of IGH haplotype diversity in antibody-mediated immunity. Hosted by Meredith Ashby, Director of Microbial Genomics at PacBio. Access additional PacBio resources for COVID-19 research at https://pacb.com/COVID-19

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PacBio Workshop: Understanding the biology of genomes with HiFi sequencing

Korlach, Jonas and Graves-Lindsay, Tina and Vierra, Michelle
PacBio; McDonnell Genome Institute

2020

Description +

The utility of new highly accurate long reads, or HiFi reads, was first demonstrated for calling all variant types in human genomes. It has since been shown that HiFi reads can be used to generate contiguous, complete, and accurate human genomes, even in repeat structures such as centromeres and telomeres. In this virtual workshop scientists from PacBio as well as Tina Graves-Lindsay from the McDonnell Genome Institute at Washington University share the many improvements we’ve made to HiFi sequencing in the past year, tools that take advantage of HiFi data for variant detection and assembly, and examples in numerous genomics application areas, including the high-quality assemblies of even the largest and most complex genomes, such as the California Redwood tree genome.

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Customer Experience: A new view of non-coding RNA with PacBio HiFi reads

Kuo, Richard
Roslin Institute, University of Edinburgh

2020

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Richard Kuo’s research at the Roslin Institute exploring non-coding RNA of avian species requires high accuracy. SMRT Sequencing on the PacBio Sequel II System and the Iso-Seq method have given him confidence in annotations across entire genomes and transcriptomes.

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Customer Experience: HiFi reads help scientists explore genomes and transcriptomes

Schmutz, Jeremy and Conesa, Ana and Gharbi, Karim and Kuo, Richard
HudsonAlpha Institute for Biotechnology; University of Florida; Earlham Institute; University of Edinburgh

2020

Description +

Highly accurate long reads, known as HiFi reads, are a new tool in scientists’ sequencing toolbox. Hear PacBio users share how they are using HiFi reads to explore the genomes, transcriptomes, metagenomes and the benefits HiFi reads provide for their addressing critical life science questions.

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Customer Experience: Improving transcriptome research with PacBio HiFi reads

Conesa, Ana
University of Florida

2020

Description +

Accurate sequencing data is key for University of Florida scientist Ana Conesa. She is using PacBio HiFi reads from the Sequel II System to identity alternative isoforms and determine the functional impact of different isoform expression in her transcriptome research.

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Webinar: Long HiFi reads for high-quality genome assemblies

Korlach, Jonas
PacBio

2020

Description +

In this LabRoots webinar, Jonas Korlach the CSO of PacBio provides an introduction to PacBio HiFi sequence reads, which are both long (up to 25 kb currently) and accurate (>99%) at the individual single-molecule sequence read level andhave allowed for advances in de novo genome assemblies. Korlach reviews the characteristics of HiFi read data obtained with the Sequel II System, followed by examples of high-quality genome assemblies for human, plant and animal genomes including the different aspects of evaluating genome assemblies (contiguity, accuracy, completeness and allelic phasing) and illustrates their high quality by examples of resolving centromeres, telomeres, segmental duplications and other previous difficult to resolve regions. Korlach also presents on advances with regard to reducing the DNA input requirements (down to 5 ng for some of the presented examples) to allow for the sequencing of single individuals of small organisms, and summarize the latest advances in using full-length RNA sequencing using the Iso-Seq method for genome annotation.

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Webinar: Opportunities for using PacBio long-read sequencing for COVID-19 research

Ashby, Meredith
PacBio

2020

Description +

In this Labroots webinar, Meredith Ashby, Director of Microbial Genomics at PacBio, describes the utility of highly accurate long-read sequencing, known as HiFi sequencing, to understand the SARs-CoV-2 viral genome. HiFi sequencing enables mutation phasing and rare variant detection to understand viral stability and mutation rates, as well as providing insights into viral population structure for monitoring viral evolution. Ashby also shares how HiFi sequencing can be used to explore the host immune response to COVID-19, specifically by providing full-length sequencing of the B cell repertoire, IGH locus and HLA genes.

Access additional COVID-19 Sequencing Tools and Resources.

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Webinar: A HiFi view – Sequencing the gut microbiome with highly accurate long reads

Ashby, Meredith and Ma, Bing and Weinstock, George
PacBio, University of Maryland School of Medicine, The Jackson Lab

2020

Description +

In this webinar, Dr. Ashby gives attendees a brief update on PacBio’s metagenomics solutions on the Sequel II System. Then, Dr. Ma, University of Maryland School of Medicine, discusses her work using long read sequencing to identify high-resolution microbial biomarkers associated with leaky gut syndrome in premature infants. Finally, Dr. Weinstock, The Jackson Laboratory, talks about the potential of highly accurate long reads to enable strain-level resolution of the human gut microbiome by resolving intraspecies variation in multiple copies of the 16S gene.

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Webinar: Bioinformatics lunch & learn – HiFi assembly

Weber, Kay
PacBio

2020

Description +

The release of the PacBio Sequel II System in 2019 brought dramatic throughput improvements and protocols for producing a new data type, highly accurate long reads or HiFi reads. PacBio is the only sequencing technology to offer highly accurate long reads (HiFi reads) that provide Sanger-quality accuracy (>99%) with the read lengths needed for assembly of complex genomes. The long length and high accuracy of HiFi reads makes them the ideal starting point for many applications, and one area of major interest is genome assembly. HiFi assembly is faster, cheaper, more accurate, and easier to phase than standard long-read assembly. In this webcast, you will learn: The difference between long reads and HiFi reads; the strengths of HiFi read assembly for human, plant, animal, and insect genomes; step-by-step instruction on how to assemble HiFi reads using the publicly available FALCON package (https://github.com/PacificBiosciences/pb-assembly)

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Webinar: Sequencing 101 – How long-read sequencing improves access to genetic information

Mars, Kristin and Laird Smith, Melissa and Vinnere Pettersson, Olga and Hardigan, Michael
PacBio, Icahn Institute for Data Science and Genomic Technology, Uppsala University, University of California Davis

2020

Description +

In this webinar, Kristin Mars, Sequencing Specialist, PacBio, presents an introduction to PacBio’s technology and its applications followed by a panel discussion among sequencing experts. The panel discussion addresses such things as what long reads are and how are they useful, what differentiates PacBio long-read sequencing from other technologies, and the applications PacBio offers and how they can benefit scientific research.

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AGBT Presentation: Feed the World – Developing genomic resources for insects as food

Oppert, Brenda
United States Department of Agriculture

2020

Description +

In a push to develop insect-based food sources for people, Brenda Oppert from the USDA has been sequencing bug genomes with PacBio technology. Long reads are essential because of the highly repetitive sequences and large genomes. On the Sequel II System, a single SMRT Cell is sufficient to generate 350-fold coverage and produce a high-quality assembly for some of the insects she’s studying.

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AGBT Presentation: Generating high quality human reference assemblies with PacBio sequencing

Graves-Lindsay, Tina
McDonnell Genome Institute at Washington University

2020

Description +

Tina Graves-Lindsay from the McDonnell Genome Institute reports at AGBT 2020 on how her team is using PacBio sequencing to produce reference-grade human genome assemblies. With highly accurate HiFi reads, no error correction step is needed during the sequencing and analysis process, and they can produce reference-grade assemblies with half the sequence coverage needed before. They are now generating diploid assemblies and will be contributing to the human pangenome reference project.