Sequencing systems
Platforms that set the standard for accurate, scalable long-reads.
The home of HiFi sequencing
PacBio offers a portfolio of advanced and accurate long-read sequencing systems to help you make discoveries that can change the world. Whether it’s interrogating difficult genomic regions, detecting novel transcript isoforms, or scaling up to population-sized studies, our systems let you see complete data without compromise.
REVIO SYSTEM
Long-read sequencing
The Revio system with SPRQ chemistry delivers 120 Gb per SMRT Cell, ideal for large-scale projects. Its fully automated workflow runs up to 4 SMRT Cells simultaneously, maximizing efficiency and throughput.
VEGA SYSTEM
Long-read sequencing
The Vega benchtop system delivers 60 Gb per SMRT Cell —ideal for labs that value speed and simplicity. Its user-friendly interface and single SMRT Cell configuration put you in full control for streamlined, immediate sequencing.
Long-read sequencing
PacBio is the only sequencing technology to offer HiFi reads that provide accuracy of 99.9%. With HiFi reads, you no longer compromise long-read lengths for high accuracy sequencing to address your toughest biological questions.
VEGA BENCHTOP system
Built on Revio technology, the Vega benchtop system brings HiFi sequencing into your lab, giving you full control to produce the most complete genomes, transcriptomes, and epigenomes. Streamline your projects and save time by sequencing in-house. Your lab, your data, your discoveries—faster than ever.
REVIO PRODUCTION system
The Revio system revolutionized HiFi sequencing with production-scale power. Now, SPRQ chemistry pushes the limits—requiring just 500 ng of input, expanding applications, cutting genome costs, and adding 6mA on-instrument methylation calling. Get ready to push the boundaries of what’s possible.
Key differences between long-read systems
 Vega system |
 Revio system |
|
| System specifications | ||
| Input amount | 2 µg / SMRT Cell | 500 ng / SMRT Cell |
| Run times | 12 and 24-hours | 12, 24, and 30-hours |
| SMRT Cells per run | 1 SMRT Cell | 1 to 4 SMRT Cells |
| Yield per SMRT Cell | 50-60 Gb | 100-120 Gb (400-480 Gb per run) |
| Annual throughput | ||
| 200 Vega SMRT Cells | 1,300 Revio SMRT Cells | |
| Key applications |
600 full-length RNA transcriptomes (10M) |
2,500 human genomes (20X) |
Sequencing in action
Video
Stories with PacBio: People first, because that’s where hope starts
In this interview with PacBio, Laura and Dave share their family’s rare disease journey and how they navigate home life with three young boys who all have an undiagnosed condition, leaving them without the important answers they are so desperately looking for. This story is the case for so many families around the world who are challenged by undiagnosed diseases.
PacBio technology applications in the field
Our blog features voices from PacBio partners, colleagues and customers — discussing the latest research, publications, and updates about genomic sequencing.
HIFI LONG READ SEQUENCING
HiFi reads let you accurately detect all types of variants, from single nucleotide to structural variants, with high precision and recall and phase haplotypes, even in hard-to-sequence regions of the genome.
Explore datasets
Browse our collection of publicly available datasets generated with PacBio sequencing.
Explore
PacBio has over 10,000 articles, reports, papers, and videos related to genomic sequencing
Frequently asked questions
Long-read sequencing with Revio and Vega systems produce genomic data by generating individual reads that are thousands of nucleotides or more in length. These reads typically come from “native” DNA from a biological sample, preserving any base modifications present. On the other hand, most short-read sequencing technologies use fragments that are 50 to 300 bases long and rely on enzymatic amplification to generate sufficiently large clonal populations, which introduces bias and loses information about base modifications. Compared to alternative long-read approaches, HiFi sequencing excels in read accuracy and ease-of-use. Read about how PacBio HiFi long read sequencing compares to nanopore sequencing.
Short-read sequencing with the Onso system excels at needle-in-a-haystack applications like liquid biopsy research. For over a decade, the Q30 standard of next-generation sequencing accuracy has been considered “good enough,” despite its inherent limitations. The introduction of Onso short-read sequencing redefines this standard, offering extraordinarily accurate reads with an error rate of only 1 in 10,000 bases or less (a quality score of Q40+). See how Q40+ accuracy in your sequencing could mean unlocking new levels of precision and certainty in your research.
Excited to hear you’re ready to get started! From our state-of-the-art instruments to the reagents and prep kits necessary to perform HiFi and SBB sequencing, we are here to help you understand what you need to get the best results.
If you’re looking to purchase an instrument or would like to speak to someone about your project needs, fill out the form and we will be in touch soon.
Fill out the request pricing form here
Whether you are looking into purchasing your first PacBio sequencer or scaling up your fleet, we have flexible financing options for you.
- Step 1: Speak to your sales representative.
- Step 2: Apply for the plan that fits your needs.
- Step 3: Start discovering sooner.
Find out more about financing options through PacBio Capital here.