Revio system
Most affordable, complete, phased genome
HiFi reads combine long-read length with high accuracy, replacing multiple assays and confidently resolving complex regions beyond the limits of short reads and nanopore methods.
HiFi sequencing at scale, with less DNA input required
Built for population-scale studies and programs that don’t pause. Run up to ~2,500 human whole genomes per year, up to 480 Gb of HiFi reads per day, and up to 4 SMRT Cells simultaneously with configurable run times and flexible scheduling — so sequencing keeps pace with your science.
On-instrument multiomic insight, included
Every standard run delivers on-instrument 5mC and 6mA methylation calling—at no additional cost or bisulfite treatment—giving you access to genomic and epigenetic data from a single sample.
Confidence in every run, without the complexity
HiFi accuracy powered by SPRQ chemistry and Google Health DeepConsensus, paired with an easy, automation-ready workflow supported by compatible partners—so you get reliable results with minimal hands-on time.
HiFi sequencing at scale
Estonia National Biobank selects PacBio to sequence 10,000 whole genomes
HiFi data generated on the Revio system will support the EU and Estonian government-funded Center for Personalized Medicine to enable discoveries across cardiology, mental and reproductive health, drug response, cancer research and rare diseases.
PacBio and GeneDx Launch Research Collaboration with the University of Washington
This study is first of its kind to compare diagnostic rates across short- and long-read sequencing platforms to understand the potential for increased diagnostic yield in neonatal care.
Ambry Genetics and PacBio Announce Collaboration to Sequence Up to 7,000 Human Genomes
Consortium selects leading genomics companies to support pediatric Mendelian Genomics Research Center Program aimed at providing insights for families battling rare diseases.
Coming soon – SPRQ-Nx Chemistry
Revio system runs on SPRQ chemistry and helps you achieve more with less DNA – empowering you to make more discoveries faster and affordably.
New SPRQ-Nx extends SPRQ chemistry with improved consistency, new 5hmC methylation caller, and multi-use SMRT Cells, enabling a richer and cost-effective HiFi workflow. Built for projects that scale, it maintains the gold-standard HiFi quality you rely on, from population studies to routine panels.
SPRQ-NX: BUILT FOR STUDIES THAT KEEP GROWING
Webinar: Scaling HiFi Genomes and Multiomic Discovery with SPRQ-Nx
Get inspired by real-world use of SPRQ-Nx by beta customers.
SPRQ-Nx public datasets
See for yourself the latest SPRQ-Nx datasets for human, plant + animals.
Keep me informed
Be the first to hear SPRQ-Nx updates on performance and release timing.
Key applications and sample throughput with SPRQ chemistry
“HiFi long read sequencing can now produce the most comprehensive variant dataset obtainable by a single technology in a single laboratory, allowing accurate calling of substitutions, indels, STRs and SVs.”
– Kucuk et al., Genome Med, 2023
HIFI LEVEL ACCURACY AND DATA QUALITY
The Revio system with SPRQ chemistry produces exceptional read length, quality, and variant calling performance that have made HiFi sequencing so celebrated.
Reveal a more complete genome
The complete, gapless human genome marks a new era of genomics where no region of the genome is beyond your reach. In 2021, PacBio HiFi technology was used by the Telomere-to- Telomere (T2T) Consortium to fill in those gaps.
Read length
HiFi reads are tens of kilobases long, providing the ability to resolve large variants and map to difficult regions of the genome.
Read and base quality
The Revio system provides reliable answers through exceptional accuracy, with 90% of bases Q30+ and median read accuracy ≥Q30.
Variant calling
HiFi sequencing with SPRQ chemistry on the Revio system delivers high accuracy variant detection, resulting in fewer sequencing errors relative to other technologies at lower coverage1.
How Revio long-read sequencing compares
| PacBio Revio with SPRQ | SBS sequencing | Nanopore sequencing | |
|---|---|---|---|
| Read length | 15-20 kb5 | 2x150 bp | 10–100 kb |
| Read accuracy | 99.95% (Q33)1 | 99.92% (Q31)3 | 98.90% (Q19)4 |
| Run time | 24 hours2 | 44 hours | 72 hours |
| Yield | 120 - 480 Gb2 | 2,400–3,000 Gb | 50–110 Gb |
| Coverage | Unbiased | Reduced at low and high (GC) | Reduced in low-complexity runs |
| Genome completeness6 | ✓ | X | ✓ |
| Variant calling – SNVs, Indels, SVs5 | ✓ | X | X |
| Areas of high homology | ✓ | X | X |
| Phasing | ✓ | X | ✓ |
| Methylation | Every run | Via library prep | Every run |
| 1. Read accuracy PacBio HiFi: precisionFDA Truth Challenge V2 3. Illumina: HG002 2×150 bp NovaSeq library, precisionFDA Truth Challenge V2 | |||
Workflow
Extraction
Nanobind DNA kits enable extraction of HMW gDNA from a variety of samples: saliva, cultured cells, bacteria, whole blood, tissue, plant nuclei, and insect.
Library prep
SMRTbell prep kit 3.0, HiFi prep kits, and application kits (Kinnex, PureTarget) support manual and automated library prep for effortless SMRTbell library creation.
Sequencing
Built for scale and flexibility, Revio lets you run 4 SMRT Cells at once, and scheduling that fits your lab.
Data analysis
Advanced algorithms from Google Health and cutting-edge NVIDIA GPUs allow for on-board processing of sequencing data, all with reduced output file sizes.
Simplified Consumables
The Revio system simplifies the HiFi sequencing experience with fewer consumables optimized for easy tracking and minimal waste. With workflow improvements, loading consumables takes under a minute at the instrument.
Revio SMRT Cell tray
One tray contains 4 high-density SMRT Cells.
Revio SPRQ sequencing plate
The sequencing plate provides reagents for sequencing up to 4 Revio SMRT Cells.
A WORKFLOW BUILT FOR YOUR SCHEDULE
The Revio system gives you a vastly simplified experience with consumables and software designed for flexibility and convenience. Whether you’re a genome center or a small lab, you can enjoy:
Fast, reliable library preparation
Library prep for the Revio system supports manual and automated prep. Learn more
Rapid run setup
Sequencing plates are linked automatically to run designs through an NFC tag, enabling runs to be set up in less than one minute on instrument.
Flexibility to fit your schedule
The Revio work deck is isolated from the four sequencing stages, leaving the system available up to 20 hours per day for loading consumables for subsequent runs.
MASSIVE COMPUTE POWER
The Revio system includes powerful NVIDIA GPUs, which provide more than 20× the compute power of the Sequel IIe system, to deliver accurate, ready-to-use data directly from the instrument.
HiFi read generation
The GPUs provide rapid turnaround time for basecalling and HiFi read generation, directly outputting HiFi reads in BAM format.
Google Health DeepConsensus
DeepConsensus uses advanced deep learning techniques to further extend the accuracy and yield of HiFi sequencing.
Methylation calling (5mC and 6mA)
Standard runs include DNA methylation status, calculated with a deep learning algorithm that processes polymerase kinetics.
Demultiplexing
All fundamental processing steps are performed on instrument, including barcode demultiplexing.
More HiFi reads, more samples, more discovery
System specifications with SPRQ chemistry
The Revio system utilizes nanofabricated Revio SMRT Cells and has four independent stages, allowing sequencing of multiple SMRT Cells in parallel. The onboard compute provides accurate basecalling with Google DeepConsensus, plus methylation calling in every run.
| Library | Run timea | Q30+ bases | HiFi yield per SMRT Cellb | Methylation | ||||
|---|---|---|---|---|---|---|---|---|
| 0.5-5 kb | 12 hours | 95% | 6-8 million reads | 5mC at CpG sites and 6mA for native DNAc | ||||
| 5-10 kb | 24 hours | 90% | 35-70 Gb | |||||
| 10-15 kb | 70-100 Gb | |||||||
| 15-20 kb | 100-120 Gb | |||||||
| 20-25 kb | 30 hours | 85% | 100-120 Gb | |||||
| ||||||||
Instrument specifications
| Operating environment | |
| Temperature | 19–25°C (66–77°F) |
| Humidity | 20–80% relative humidity, non-condensing |
| Ventilation | 13,000 BTU/hr (3,800 W) |
| Dimensions | |
| W × D × H | 92.7 cm (36.5 in) × 91.4 cm (36.0 in) × 174.5 cm (68.7 in) |
| Weight | 465 kg (1,025 lb) |
| Floor space | 243.8 cm (96.0 in) × 138.5 cm (54.5 in) |
| Electrical power | |
| Power requirements | 200–240 VAC at 50-60 Hz, max 5 kVA/4.8 kW/24A |
| Compute | |
| Network connection | 1 GbE or 10 GbE, copper |
| Output file format | hifi_reads.bam, ~60 GB each, up to 78 TB per year |
Frequently asked questions
SPRQ (pronounced “spark”) is PacBio’s newest HiFi chemistry, designed to make sequencing more efficient and expand HiFi sequencing to more sample types and applications. It requires only 500 ng of native DNA, opening up new possibilities for challenging or precious samples. SPRQ also helps reduce genome costs by increasing HiFi yields per SMRT Cell by 33%, enabling sequencing of two human genomes at 20X coverage per SMRT Cell. In addition, SPRQ supports deeper multiomic insights into chromatin architecture with Fiber-seq, featuring on-instrument 5mC and 6mA calling for greater precision in methylation detection.
SPRQ-Nx (pronounced “next”) is an update to SPRQ chemistry that increases data consistency, new 5hmC methylation caller, and introduces a new multi-use SMRT Cell that can be used for multiple acquisitions on the Revio system. “Nx” represents that a SMRT Cell can be used “N” times and is the next evolution in HiFi sequencing.
SPRQ-Nx is currently in beta testing and will be available in Q2-2026. Want to be among the first to hear the latest news about SPRQ-Nx?
The Revio system with SPRQ chemistry sequences two human genomes at 20x coverage for just $5006. Your local sales representative can provide detailed pricing in your currency.
The capabilities† of just one Revio SMRT Cell include:
- Up to 120 Gb of total HiFi long-read sequencing data in 24 hours.
- Two phased 20x human whole genome.
- One sample with ~80 million full-length RNA isoforms using the Kinnex single-cell RNA kit.
- 16 samples using large, targeted gene panels such as the Dark Gene.
- 96 samples using small, targeted gene panels similar to the Long-read PGx.
- 16 communities of metagenome assembled genomes.
† Expected coverage and throughput are estimates. Coverage and total reads/data may vary based on library quality and fragment lengths.
The Revio system outputs ready-to-use HiFi reads in BAM format. All fundamental processing steps are performed on instrument, including barcode demultiplexing.
- Data shown is for a single Revio SMRT Cell for HG002/GM24385.
- HiFi yield specification based on HG002/GM24385 human DNA extracted with Nanobind CBB kit and prepared with SMRTbell prep kit 3.0.
- Run time specification is for the sequencing reaction.
- Expected coverages and throughputs are estimates. Coverage may vary based on library quality and fragment lengths.
- HiFi yield is dependent on library fragment size. Yield is typically lower for shorter libraries.
- Currency is calculated based on reagent list pricing in USD.
See system specification sheet for more details.
EXPLORE THE REVIO SYSTEM
Take a 3D tour of the Revio system to see key features, setup, workflows or request a quote.