Now with SPRQ-Nx
The first HiFi benchtop system – bringing industry-leading and exceptionally accurate long reads to your lab.
- Lower DNA input as little as 500 ng
- Yield increase to 90 Gb per run
- Same day results with 2- and 4-hour run times
- Supports 21 CFR Part 11 compliance
- Additional methylation biomarkers 5mC, 5hmC, 6mA in every run
Premium HiFi data, accessibly priced
Priced at USD $189,0001 and $995 per run, Vega delivers 90 Gb of long reads per run, now unlocking applications and sample types available.
Sequence your way in house
Manage project times more effectively by sequencing in-house and control your timelines from start to finish with low input and single-shift movies.
Worry-free workflows
Adopting HiFi sequencing is simpler than ever with the PacBio universal HiFi workflow, from extraction to data analysis. Combined with intuitive run setup, the Vega system enables laboratories to consolidate several multiomics applications onto a single sequencing platform, simplifying workflows while delivering reliable, consistent HiFi results.
21 CFR Part 11 compliance
Built-in features for 21 CFR Part 11 compliance through audit tracking and system login to support in regulated environments.
1Price effective October 1, 2026.
Industry-leading long-read technology
HiFi sequencing delivers 99.9% read accuracy, enabling you to achieve long read lengths without sacrificing accuracy to address complex biological questions.
With over 1,350 peer-reviewed publications in 2025 alone and more than 1,200 PacBio sequencers globally, Vega now has SPRQ-Nx chemistry, the same chemistry as on the Revio platform
What is HiFi sequencing? How it works
Turn your lab into tomorrow's discovery hotspot
One Vega SMRT Cell means…
8 full-length RNA transcriptomes
96 PureTarget repeat expansion panels
384 microbial genomes
And more…
Application brief
21 CFR Part 11 on Vega + SMRT Link
Vega software provides the core technical controls needed to help support 21 CFR Part 11 compliance, including user authentication, audit trails, and access management.
- User authentication: Optional login mode requires users to authenticate with their SMRT Link credentials, extending accountability to on-instrument activity.
- Audit trails: Comprehensive, tamper-evident audit trails record who performed each action, what was done, and when, with administrator access and export capabilities for documentation and review.
WHAT CAN VEGA LONG READS DO FOR YOU?
Target what matters
Targeted HiFi sequencing allows you to sequence only the genomic regions you care about – easily, and cost-effectively. The length and accuracy of HiFi reads combined with deep coverage is a powerful approach to gain new information and resolve “challenging” regions often missed by short reads.
Complete view of isoform repertoire with Kinnex
Elevate your research with highly accurate full-length RNA information. Kinnex with the Vega system offers full-length isoform discovery and abundance information, and cell type-specific isoform discovery, that are difficult to capture with short reads.
Comprehensive genetic insights from telomere to telomere
HiFi long-read genomes deliver all variants including difficult-to-detect variants, structural variation and methylation changes involved in diseases that are challenging or impossible to detect with short reads. See data comparisons between Vega and Revio systems.
Biopharma
HiFi sequencing plays an increasingly important role in drug discovery and development. Whether it’s identifying biomarkers, engineering mRNA or oligonucleotide therapeutics, optimizing AAV vectors, developing antibodies, or characterizing immune repertoires, accurate and complete data is essential.
Shrink your costs, grow your ambitions
Whether you are purchasing your first PacBio sequencer or scaling up your fleet, contact a sales rep to see what Vega can bring to your lab. With a reagent commitment, Vega Access offers an especially affordable purchasing option.
Price shown in US dollar and may differ by region. Contact a PacBio sales rep for all pricing options in your currency.
Small and mighty
- Innovative Revio technology shrunk to fit on a benchtop – equivalent HiFi data in a reduced instrument footprint.
- Seamless integration with HiFi library prep workflows and SMRT Link run management software
. - On-instrument primary analysis, including DeepConsensus, methylation calling, and optional barcode demultiplexing in every run.
- Built-in features to help support 21 CFR 11 compliance in regulated environments, including system login and audit tracking.
Two consumables, too easy
The Vega SMRT Cell is the heart of HiFi sequencing and is powered by a semiconductor device to deliver single-molecule insights in real time.
The Vega sequencing plate holds everything you need – sample and reagents – ready to power your next game-changing insight.
A push-button run setup links the Vega sequencing plate to SMRT Link run design. Intuitive design and tactile confirmations reduce errors and steps to get to results.
No fuss, just functionality with standard power and networking for a true plug-and-play experience.
Sequence your way
A single SMRT Cell per run means no waiting, putting you in control of your data, timelines, and budget. By sequencing in-house, you can streamline your workflows, finish projects faster, and tailor every run to your application needs—all with an instrument designed to fit your lab and your experiments.
- Continuous productivity: Vega lets you pre-stage consumables for the next run—keeping you one step ahead and boosting efficiency.
- Shorter run times: Short movie times enable single-shift workflows, improving lab efficiency and turnaround time.
Design + control experiments with SMRT Link software
Web-based SMRT Link software supports sequencing prep, run design, and data analysis in a single interface across a wide range of applications.
Vega transfers sequencing data to your preferred network or directly to Amazon, Google, or Microsoft cloud.
SMRT Analysis provides fast, easy, and powerful computational tools and workflows to get complete views of the full genome or specific region of interest and exceptionally accurate variant calling like TRGT, and more.
Save on compute and storage costs
The Vega system goes beyond basecalling – every run also measures DNA methylation status, calculated with a deep learning algorithm. On-instrument primary analysis includes barcode demultiplexing and conversion to the standard BAM format – so you can make meaningful insights faster. Efficient data representation encodes sequence, quality values, and methylation in about 0.5 bytes per base — 20× smaller than for other long-read technologies.
| Technology | On instrument | Off instrument | Typical output file format and size |
 Vega system |
|
| hifi_reads.bam Vega: 45 GB |
 Other long-read technology |
|
| Fast5/pod5 1,300 GB |
Worry-free and flexible workflows so you can start right away.
- Universal HiFi workflow from extraction to data with options for manual and automated solutions for all PacBio systems.
- 30+ PacBio Compatible partners provide seamless integration of kits and analysis solutions to support customers who are just starting out with HiFi or expanding their current capabilities. Learn more
- World class service and support teams. With our dedicated support teams by your side and the Customer Hub as your one-stop online solution, managing orders and getting assistance has never been easier.
Get a more complete view of biology with HiFi reads
More comprehensive variant calling with phasing + 5mC, 5hmC, and 6mA methylation
Access high accuracy for all variant types — SNVs, indels, structural variants, tandem repeat expansions, and methylation — including in challenging regions.1
Full-length isoform sequencing
Characterize complex alternative splicing, fusion transcripts, prediction of open reading frames, and cell-type specific isoform expression.
Targeted sequencing to study genes
Focus the power of HiFi variant calling by enriching for regions of interest using PureTarget, hybrid capture or PCR amplification.
Microbial genomics
Characterize, assemble, and gain a better understanding of microbial communities and metagenomics
Frequently asked questions
HiFi sequencing is a single-molecule long-read technology that produces highly accurate, long DNA reads by combining multiple passes of the same molecule into a consensus sequence, allowing you to resolve complex genomic regions and close gaps in your data.
SPRQ-Nx is an updated HiFi sequencing chemistry for the Vega system that enables reduced sequencing costs, increased yields, and support for 21 CFR part 11 compliance. It adds 5hmC methylation detection, improves 5mC and 6mA detection performance, and delivers more consistent yield and accuracy across runs.
Yes, there are publicly available Vega datasets. Discover what Vega can do – download and explore these datasets now:
- Full-length isoform discovery with Kinnex and Vega dataset.
- Interrogate repeat expansions with PureTarget and Vega dataset.
- See HiFi genome data comparisons between HiFi systems.
If you’re already doing HiFi sequencing, transitioning to the Vega system is seamless. Your workflow stays nearly the same, with only a slight change in sequencing consumable kits. Just follow the naming guidelines, and you’re ready to sequence!
From Nanobind sample prep kits to HiFi plex library prep kits and Kinnex application kits, your current kits are fully compatible with Vega. Plus, the same HiFi analysis tools you trust with Revio work perfectly with Vega too. Expect the same high performance, reliability, and confidence.
HiFi long-read sequencing generates individual reads that are thousands of nucleotides long, using “native” DNA from your sample. This preserves important base modifications, so you automatically get methylation detection included in every run.
In contrast, short-read sequencing uses much shorter fragments (50 to 300 bases) and requires enzymatic amplification, which can introduce bias and miss base modifications. This makes it harder for short reads to handle complex regions, like repetitive areas or isoform rearrangements. Learn about what sets HiFi reads apart in our HiFi Difference blog series.
Compared to other long-read methods, HiFi sequencing stands out for its accuracy and simplicity. Read about how PacBio HiFi long read sequencing compares to nanopore sequencing in our blog.
1Price effective October 1, 2026. Prices may differ by region. Contact PacBio sales rep for pricing in your currency.
Pushing boundaries, not space