Understand how PacBio HiFi sequencing delivers 99.9% accuracy and native epigenetic data by reading a single molecule repeatedly. This animation visualizes the complete SMRT sequencing mechanics, from library prep to data analysis. The process relies on circularizing DNA templates to observe the same native strand multiple times, eliminating the need for PCR amplification. By bypassing this amplification step, the technology resolves complex GC-rich “dark” regions and captures subtle kinetic signatures to identify methylation, providing a comprehensive multiomic view in a single run. The HiFi difference
- Achieve greater than 99.9% long-read accuracy
- Eliminate PCR amplification bias to resolve GC-rich and repetitive regions
- Capture comprehensive multiomics data from a single native molecule.
- Resolve structural variants and full-length isoforms without bias.