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Procedure & checklist – Preparing Kinnex libraries using the Kinnex 12-fold prep kit

Description

This procedure describes the workflow for constructing Kinnex 12-fold libraries from cDNA generated using Iso-Seq express 2.0 kit, 16S rRNA amplicons, or other types of Kinnex-compatible amplicons. The generated libraries can be sequenced on PacBio Vega and Revio systems.

Procedure & checklist – Preparing Kinnex libraries using the Kinnex 16-fold prep kit

Description

This procedure describes the workflow for constructing Kinnex 16-fold libraries from single-cell cDNA or from amplicons with a peak size of 0.5 – 1 kb. The generated libraries can be sequenced on PacBio Vega and Revio systems.

Procedure & checklist – Preparing Kinnex libraries using the Kinnex 8-fold prep kit

Description

This procedure describes the workflow for constructing Kinnex 8-fold libraries from cDNA generated using Iso-Seq express 2.0 kit, 16S rRNA amplicons, or other types of Kinnex-compatible amplicons. The generated libraries can be sequenced on PacBio Vega and Revio systems.

Procedure & checklist — Preparing Kinnex-compatible cDNA using Iso-Seq express 2.0 kit

Description

This procedure describes the workflow for generating cDNA from polyadenylated (polyA) RNA using the Iso-Seq express 2.0 kit. The resulting cDNA can be used for either (1) generation of Kinnex concatenated libraries using Kinnex concatenation kit 2.0 or (2) construction of Iso-Seq monomer libraries using SMRTbell prep kit 3.0. The libraries are compatible with the PacBio Vega and Revio systems.

Guide & overview — Automated HiFi plex prep 96 for the Hamilton NGS STAR MOA system

Description

This Guide & Overview describes the workflow for PacBio high-throughput semi-automated long read sample prep utilizing the HiFi plex prep 96 kit on the Hamilton NGS Microlab STAR MOA system. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.

Guide & overview — Automated-HiFi-prep-96-and-HiFi-ABC-for-the-Hamilton-NGS-STAR-MOA-system

Description

This Guide & Overview describes the high throughput automated workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96 on the Hamilton NGS Microlab STAR MOA system.

Procedure & checklist – Generating PureTarget libraries with PureTarget kit 96 automation protocol

Description

This procedure describes the workflow for generating PureTarget repeat expansion or carrier libraries.

Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing

Description

This procedure describes the workflow for constructing SMRTbell libraries with PCR amplification from as little as 1 ng of starting genomic DNA using the Ampli-Fi protocol. Sequencing libraries prepared with this procedure are compatible with PacBio Revio and Vega systems.

Procedure & checklist — Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems. Amplicons may be indexed during PCR, or during library preparation with SMRTbell indexed adapters.

Procedure & checklist — Preparing multiplexed whole genome and amplicon libraries using the HiFi plex prep kit 96

Description

This procedure describes the workflow for constructing multiplexed whole genome sequencing (WGS) and amplicon libraries using the HiFi plex prep kit 96 for sequencing on PacBio HiFi systems. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.

Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems.

Guide & overview – Automated PureTarget kit 96 for the Hamilton NGS STAR MOA system

Description

This procedure describes the automated workflow for generating PureTarget libraries using the PureTarget kit 96 on the Hamilton NGS STAR MOA system. The kit is designed for 96 samples per automated run.

Procedure & checklist – Generating PureTarget libraries with PureTarget kit 24 manual protocol

Description

This procedure describes the workflow for manually generating PureTarget libraries.

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio HiFi systems.

Procedure & Checklist — Preparing SMRTbell Libraries using PacBio Barcoded M13 Primers for Multiplex SMRT Sequencing

Description

This procedure can be used to generate asymmetrically barcoded PCR products for library construction and SMRT sequencing by amplifying the region of interest using target-specific PCR primers tailed with M13 forward and reverse primers.