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Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems.

Procedure & checklist – Generating PureTarget libraries with PureTarget kit 24 manual protocol

Description

This procedure describes the workflow for manually generating PureTarget libraries.

Procedure & checklist – Generating PureTarget libraries with PureTarget kit 96 automation protocol

Description

This procedure describes the workflow for generating PureTarget repeat expansion or carrier libraries.

Guide & overview — Short Read Eliminator SRE DNA shearing and cleanup for the Hamilton Microlab Prep system

Description

This Guide & overview describes the sample, reagent, and consumable preparation for Short Read Eliminator (SRE), DNA shearing, and post-shear cleanup on the Hamilton Microlab Prep (MLP) Liquid Handling system. This Guide & overview is meant to be used with the HiFi prep kit 96, HiFi plex prep kit 96, or SMRTbell® prep kit 3.0 library protocols.

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio HiFi systems.

Guide & overview – Automated PureTarget kit 96 for the Hamilton NGS STAR MOA system

Description

This procedure describes the automated workflow for generating PureTarget libraries using the PureTarget kit 96 on the Hamilton NGS STAR MOA system. The kit is designed for 96 samples per automated run.

Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing

Description

This procedure describes the workflow for constructing SMRTbell libraries with PCR amplification from as little as 1 ng of starting genomic DNA using the Ampli-Fi protocol. Sequencing libraries prepared with this procedure are compatible with PacBio Revio and Vega systems.

SMRT Link Kinnex single-cell troubleshooting guide v13.1

Description

This document describes the metrics generated by the Read Segmentation and Single-Cell Iso-Seq workflow in SMRT Link v13.1. The document also describes possible issues that can occur when using the Kinnex single-cell RNA kit for both supported and unsupported use cases.

Procedure & checklist — Preparing Iso-Seq libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing Iso-Seq libraries using SMRTbell prep kit 3.0 from RNA for sequencing on PacBio HiFi systems

Procedure & checklist — Preparing Kinnex libraries using the Kinnex full-length RNA kit

Description

This procedure describes the workflow for constructing Kinnex full-length RNA libraries from total RNA samples for sequencing on PacBio HiFi systems.

Procedure & checklist — Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems. Amplicons may be indexed during PCR, or during library preparation with SMRTbell indexed adapters.

Procedure & checklist — Preparing multiplexed whole genome and amplicon libraries using the HiFi plex prep kit 96

Description

This procedure describes the workflow for constructing multiplexed whole genome sequencing (WGS) and amplicon libraries using the HiFi plex prep kit 96 for sequencing on PacBio HiFi systems. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.

Procedure & checklist — Preparing whole genome libraries using the HiFi prep kit 96

Description

This procedure describes the high throughput workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96.

Guide & overview — Nanobind HT kits

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the Hamilton NIMBUS Presto system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.