Featured Documentation

Guide & overview – Automated 3.1x diluted AMPure PB for saliva gDNA on the Hamilton NGS STAR MOA system

Description

This procedure describes the automated workflow for performing 3.1x dilute AMPure® PB bead cleanup on saliva gDNA using the Hamilton NGS STAR MOA system. This step is necessary to improve coverage of saliva samples for PureTarget

Guide & overview – Automated PureTarget kit 96 for the Hamilton NGS STAR MOA system

Description

This procedure describes the automated workflow for generating PureTarget libraries using the PureTarget kit 96 on the Hamilton NGS STAR MOA system. The kit is designed for 96 samples per automated run.

Guide & overview — Automated-HiFi-prep-96-and-HiFi-ABC-for-the-Hamilton-NGS-STAR-MOA-system

Description

This Guide & Overview describes the high throughput automated workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96 on the Hamilton NGS Microlab STAR MOA system.

Guide & overview — Short Read Eliminator SRE DNA shearing and cleanup for the Hamilton Microlab Prep system

Description

This Guide & overview describes the sample, reagent, and consumable preparation for Short Read Eliminator (SRE), DNA shearing, and post-shear cleanup on the Hamilton Microlab Prep (MLP) Liquid Handling system. This Guide & overview is meant to be used with the HiFi prep kit 96, HiFi plex prep kit 96, or SMRTbell® prep kit 3.0 library protocols.

Guide & overview — Nanobind HT kits

Guide & overview — Automated HiFi plex prep 96 for the Hamilton NGS STAR MOA system

Description

This Guide & Overview describes the workflow for PacBio high-throughput semi-automated long read sample prep utilizing the HiFi plex prep 96 kit on the Hamilton NGS Microlab STAR MOA system. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.

Guide & overview — Nanobind CBB kit

Description

This document describes procedures on how to use the Nanobind CBB Kit for extraction of HMW (50 kb – 300+ kb) DNA from cultured cells, cultured bacteria, and blood.

Guide & overview — Nanobind PanDNA kit

Description

This guide and overview describes the PacBio® Nanobind PanDNA kit which can be used for diverse sample types including cultured cells and bacteria, human blood and tissue, animal blood and tissue, insects, and plant nuclei. For each sample type, a detailed Procedure & Checklist can be found at the PacBio Documentation page.

Guide & overview — Short Read Eliminator (SRE) XS and XL kits

Description

This document describes procedures on how to use the the SRE kit family (SRE XS, SRE XL) for rapid high-pass size selection of high molecular weight (HMW) DNA samples.

Guide & overview — Nanobind tissue kit

Description

This document describes procedures on how to use the Nanobind tissue kit for extraction of HMW (50 kb – 300+ kb) and UHMW (50 kb – 1+ Mb) DNA from animal tissue samples.