Discover the diversity that drives adaptive immunity
From full-length T-cell receptor (TCR) and B-cell receptor (BCR) repertoires to HLA typing, HiFi sequencing enables researchers to uncover novel alleles, structural variants, and cell-type-specific expression patterns missed by other sequencing technologies. These insights are powering discoveries in vaccine development, autoimmune disease research, cancer immunotherapy, and transplant matching. With exceptional read accuracy and the ability to resolve complex regions of the genome and transcriptome, PacBio HiFi sequencing provides the clarity needed to advance precision immunology and improve human health.
HLA sequencing
Targeted sequencing to generate fully phased HLA haplotypes without imputation with HiFi reads
RNA sequencing
Bulk and single-cell whole transcriptome sequencing to characterize the complete landscape of isoform diversity, including TCR and BCR repertoire
EXPLORE IMMUNOLOGY WEBINARS
Precision immunogenic profiling in cancer with HiFi sequencing
In this webinar, learn how HiFi-empowered immunogenomic profiling can identify variants linked to therapeutic response and immune-related adverse events (IRAEs). These insights can advance biomarker discovery, inform drug development, and move us closer to more precise and effective cancer immunotherapies.
HiFi sequencing and immune diversity in vaccine response
In this PRISM recorded talk, Melissa Smith, CEO of Clareo Biosciences, explains how HiFi sequencing is essential for understanding the vast genetic diversity in human immune repertoires. This detailed characterization provides critical insights into vaccine efficacy and autoimmune disease by uncovering genetic variation previously missed by other technologies.
Understanding full-length single-cell RNA-seq of immune cells in cardiovascular diseases
In this webinar, Anastasiya Grinko, MSc, applied HiFi sequencing to profile the single-cell isoform landscape of monocyte-derived macrophages after myocardial infarction. This approach captured thousands of distinct isoforms, making it possible to see cell-type-specific isoform utilization in monocytes and macrophage subtypes and to unveil temporal dynamics of isoform expression.
Blog
The HiFi difference – immunology with a FLAIRR
Antibodies are the work horses of the adaptive immune system, identifying and neutralizing foreign objects like bacteria and viruses, and thereby are central to the science of immunology. A new preprint by Dr. Melissa Smith & colleagues from the University of Louisville, entitled FLAIRR-seq: A novel method for single molecule resolution of near full-length immunoglobulin heavy chain repertoires, describes a new gold standard to characterize expressed antibody repertoires.
Publication highlight
Targeted IGH germline sequencing reveals population diversity
Targeted human immunoglobulin heavy chain (IGH) allele sequencing of 154 healthy individuals of diverse ancestry identified novel structural variants and alleles missed by short reads, providing a foundation of studying population-level IGH variation.
Customer success story
Helping to improve bone marrow transplant outcomes with better HLA typing
Hear researchers describe how they have used PacBio sequencing for HLA typing research in the context of bone marrow transplant donor matching.
Watch the video Read the blog Read the paper
