September 22, 2019  |  

The standard operating procedure of the DOE-JGI Microbial Genome Annotation Pipeline (MGAP v.4).

Authors: Huntemann, Marcel and Ivanova, Natalia N and Mavromatis, Konstantinos and Tripp, H James and Paez-Espino, David and Palaniappan, Krishnaveni and Szeto, Ernest and Pillay, Manoj and Chen, I-Min A and Pati, Amrita and Nielsen, Torben and Markowitz, Victor M and Kyrpides, Nikos C

The DOE-JGI Microbial Genome Annotation Pipeline performs structural and functional annotation of microbial genomes that are further included into the Integrated Microbial Genome comparative analysis system. MGAP is applied to assembled nucleotide sequence datasets that are provided via the IMG submission site. Dataset submission for annotation first requires project and associated metadata description in GOLD. The MGAP sequence data processing consists of feature prediction including identification of protein-coding genes, non-coding RNAs and regulatory RNA features, as well as CRISPR elements. Structural annotation is followed by assignment of protein product names and functions.

Journal: Standards in genomic sciences
DOI: 10.1186/s40793-015-0077-y
Year: 2015

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