September 22, 2019  |  

The sea lamprey germline genome provides insights into programmed genome rearrangement and vertebrate evolution.

Authors: Smith, Jeramiah J and Timoshevskaya, Nataliya and Ye, Chengxi and Holt, Carson and Keinath, Melissa C and Parker, Hugo J and Cook, Malcolm E and Hess, Jon E and Narum, Shawn R and Lamanna, Francesco and Kaessmann, Henrik and Timoshevskiy, Vladimir A and Waterbury, Courtney K M and Saraceno, Cody and Wiedemann, Leanne M and Robb, Sofia M C and Baker, Carl and Eichler, Evan E and Hockman, Dorit and Sauka-Spengler, Tatjana and Yandell, Mark and Krumlauf, Robb and Elgar, Greg and Amemiya, Chris T

The sea lamprey (Petromyzon marinus) serves as a comparative model for reconstructing vertebrate evolution. To enable more informed analyses, we developed a new assembly of the lamprey germline genome that integrates several complementary data sets. Analysis of this highly contiguous (chromosome-scale) assembly shows that both chromosomal and whole-genome duplications have played significant roles in the evolution of ancestral vertebrate and lamprey genomes, including chromosomes that carry the six lamprey HOX clusters. The assembly also contains several hundred genes that are reproducibly eliminated from somatic cells during early development in lamprey. Comparative analyses show that gnathostome (mouse) homologs of these genes are frequently marked by polycomb repressive complexes (PRCs) in embryonic stem cells, suggesting overlaps in the regulatory logic of somatic DNA elimination and bivalent states that are regulated by early embryonic PRCs. This new assembly will enhance diverse studies that are informed by lampreys' unique biology and evolutionary/comparative perspective.

Journal: Nature genetics
DOI: 10.1038/s41588-017-0036-1
Year: 2018

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