Members of the family Vibrionaceae are metabolically versatile and ubiquitous in natural environments, with extraordinary genome feature of two chromosomes. Here we reported the complete genome of Paraphotobacterium marinum NSCS20N07D(T), a recently described novel genus-level species in the family Vibrionaceae. It contained two circular chromosomes with a size of 2,593,992 bp with G+C content of 31.2 mol%, and a plasmid with a size of 5,539 bp. The larger chromosome (Chr. I) had a genome size of 1,426,504 bp with G+C content of 31.6 mol%, and the smaller one (Chr. II) had a genome size of 1,161,949 bp with G+C content of 30.8 mol%. The two chromosomes have strikingly similar G+C contents with difference of <1% and similar percentages of coding regions. interestingly, by comparison to 134 species affiliated with seven genera within the family vibrionaceae, p. marinum nscs20n07d(t) possessed smallest genome size lowest g+c content. clusters orthologous groups proteins functional categories revealed that two chromosomes had different distributions classes, indicating they take cellular functions. surprisingly, chr. ii a large proportion unknown genes than i. metabolic characteristics predicted i performed essential metabolism, which can be complemented ii, such as amino acids biosynthesis. microbial community analysis in situ surface seawater accounted for one four sequences among more 20,000 16s ribosomal rna gene v4 contigs, representing it apparently appeared rare species. what's more, was anticipated specific pelagic ocean. this study will provide new insight into understanding genomic features multiple prokaryote emphasize ecological distribution members vibrionaceae species.
Journal: Frontiers in microbiology
DOI: 10.3389/fmicb.2017.01994
Year: 2017