Three mesophilic, Gram-stain-positive, aerobic bacterial strains, designated Uno3T, Uno11T and Uno16T, were isolated from a soil-like granular micro-organism mass (termed Tengu-no-mugimeshi) collected from Tsumagoi, Gunma, Japan. They grow at 11-37?°C?and pH 4.0-8.0, form branched mycelia, and have a G+C?content between 49.4-50.3?mol%. The major menaquinone and fatty acid of Uno3T are MK-9 and iso-C16?:?0, respectively, whereas Uno11T and Uno16T share MK-9 (H2) and C16?:?1-2OH. The major cell-wall sugars are mannose (Uno3T and Uno11T) and glucose (Uno16T). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these three strains belong to the order Ktedonobacterales and are most closely related to Dictyobacter aurantiacus S-27T (sequence similarity of 91.3, 96.4 and 95.5?%). Average nucleotide identity values were <79.9?% among uno11t, uno16t and d. aurantiacus s-27t, well below the 95-96?%?species circumscription threshold. based on phenotypic features phylogenetic positions, we propose that uno3t represents a novel genus species, tengunoibacter tsumagoiensis gen. nov., sp. nov. (type strain 113152t=LMG 30471t=BCRC 81113t) within new family dictyobacteraceae fam. strains uno11t are also considered to represent species: dictyobacterkobayashii 113153t=LMG 30472t=BCRC 81114t) dictyobacteralpinus 113154t=BCRC 81115t). an emended description of dictyobacter, classifying it dictyobacteraceae, provide descriptions genera dictyobacter ktedonobacter.
Journal: International journal of systematic and evolutionary microbiology
DOI: 10.1099/ijsem.0.003396
Year: 2019