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Authors: Chen, Yujiao and Wu, Yuqian and Liu, Li and Feng, Jianhua and Zhang, Tiancheng and Qin, Sheng and Zhao, Xingyu and Wang, Chaoxia and Li, Dongmei and Han, Wei and Shao, Minghui and Zhao, Ping and Xue, Jianfeng and Liu, Xiaomin and Li, Hongjie and Zhao, Enwei and Zhao, Wen and Guo, Xijie and Jin, Yongfeng and Cao, Yaming and Cui, Liwang and Zhou, Zeqi and Xia, Qingyou and Rao, Zihe and Zhang, Yaozhou

The complete genome of Cordyceps militaris was sequenced using single-molecule real-time (SMRT) sequencing technology at a coverage over 300×. The genome size was 32.57?Mb, and 14 contigs ranging from 0.35 to 4.58?Mb with an N50 of 2.86?Mb were assembled, including 4 contigs with telomeric sequences on both ends and an additional 8 contigs with telomeric sequences on either the 5' or 3' end. A methylome database of the genome was constructed using SMRT and m4C and m6A methylated nucleotides, and many unknown modification types were identified. The major m6A methylation motif is GA and GGAG, and the major m4C methylation motif is GC or CG/GC. In the C. militaris genome DNA, there were four types of methylated nucleotides that we confirmed using high-resolution LCMS-IT-TOF. Using PacBio Iso-Seq, a total of 31,133 complete cDNA sequences were obtained in the fruiting body. The conserved domains of the nontranscribed regions of the genome include TATA boxes, which are the initial regions of genome replication. There were 406 structural variants between the HN and CM01 strains, and there were 1,114 structural variants between the HN and ATCC strains.

Journal: Scientific reports
DOI: 10.1038/s41598-018-38021-4
Year: 2019

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