Methicillin-resistant Staphylococcus aureus (MRSA) may cause potentially lethal infections. Increasing evidence suggests that the gut microbiota is associated with human health. Yet, whether patients with MRSA infections carry specific signatures in their fecal microbiota composition has not been determined. Thus, this study aimed to compare the fecal microbiota profile of MRSA-positive patients (n=15) with individuals without MRSA infection (n=15) by using the PacBio single molecule, real-time (SMRT) DNA sequencing system and real-time quantitative polymerase chain reaction (qPCR). Mann-Whitney tests and unweighted UniFrac principal coordinate analysis (PCoA) showed that the profile of fecal microbiota was apparently different between the two populations. Both the community richness and diversity were reduced in the MRSA-positive group (p<0.050). the genera acinetobacter and enterococcus were highly enriched in mrsa-positive group, whereas less short-chain fatty acid (scfa)-producing bacteria, including butyricimonas, faecalibacterium, roseburia, ruminococcus, megamonas phascolarctobacterium, detected group. at species level, baumannii bacteroides thetaiotaomicron prevalent opposite trends observed 17 other species, such as faecalibacterium prausnitzii, lactobacillus rogosae, rupellensis phascolarctobacterium faecium. positive correlations between erythrocyte sedimentation rate (esr) (r=0.554, p=0.001), well hypersensitive c reactive protein (hscrp) prausnitzii was negatively associated with esr hscrp total bile acids (tba) conclusion, fecal microbiota structure different -negative patients. increase potential pathogens reduction of beneficial populations, scfa-producing patients may affect prognosis.
Journal: European journal of clinical microbiology & infectious diseases
DOI: 10.1007/s10096-017-2955-2
Year: 2017