April 21, 2020  |  

Next generation sequencing characterizes HLA diversity in a registry population from the Netherlands.

Authors: Hou, Lihua and Enriquez, Elizabeth and Persaud, Misti and Steiner, Noriko and Oudshoorn, Machteld and Hurley, Carolyn Katovich

Next generation DNA sequencing is used to determine the HLA-A, -B, -C, -DRB1, -DRB3/4/5, and -DQB1 assignments of 1009 unrelated volunteers for the unrelated donor registry in The Netherlands. The analysis characterizes all HLA exons and introns for class I alleles; at least exons 2 to 3 for HLA-DRB1; and exons 2 to 6 for HLA-DQB1. Of the distinct alleles present, there are 229 class I and 71 class II; 36 of these alleles are novel. The majority (approximately 98%) of the cumulative allele frequency at each locus is contributed by alleles that appear three or more times. Alleles encoding protein variation outside of the antigen recognition domains are 0.6% of the class I assignments and 5.3% of the class II assignments. © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Journal: HLA
DOI: 10.1111/tan.13535
Year: 2019

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