Multiplatform next-generation sequencing identifies novel RNA molecules and transcript isoforms of the endogenous retrovirus isolated from cultured cells.

Authors: Moldován, Norbert and Szucs, Attila and Tombácz, Dóra and Balázs, Zsolt and Csabai, Zsolt and Snyder, Michael and Boldogkoi, Zsolt

In this study, we applied short- and long-read RNA sequencing techniques, as well as PCR analysis to investigate the transcriptome of the porcine endogenous retrovirus (PERV) expressed from cultured porcine kidney cell line PK-15. This analysis has revealed six novel transcripts and eight transcript isoforms, including five length and three splice variants. We were able to establish whether a deletion in a transcript is the result of the splicing of mRNAs or of genomic deletion in one of the PERV clones. Additionally, we re-annotated the formerly identified RNA molecules. Our analysis revealed a higher complexity of PERV transcriptome than it was earlier believed.© FEMS 2018. All rights reserved. For permissions, please e-mail: [email protected].

Journal: FEMS microbiology letters
DOI: 10.1093/femsle/fny013
Year: 2018

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