Selection of highly productive algal strains is crucial for establishing economically viable biomass and biopro- duct cultivation systems. Characterization of algal genomes, including understanding strain-specific differences in genome content and architecture is a critical step in this process. Using genomic analyses, we demonstrate significant differences between three strains of Chlorella sorokiniana (strain 1228, UTEX 1230, and DOE1412). We found that unique, strain-specific genes comprise a substantial proportion of each genome, and genomic regions with> 80% local nucleotide identity constitute <15% of each genome among the strains, indicating substantial strain specific evolution. furthermore, cataloging meiosis and other sex-related genes in c. sor- okiniana strains suggests strategic breeding could be utilized to improve biomass bioproduct yields if a sexual cycle can characterized. finally, preliminary investigation epigenetic machinery pre- sence potentially unique transcriptional regulation strain. our data demonstrate that these three sorokiniana represent significantly different genomic content. based on findings, we propose in- dividualized assessment for potential performance cultivation systems.
Journal: Algal research
DOI: 10.1016/j.algal.2018.09.012
Year: 2018