Genome sequence and analysis of the Japanese morning glory Ipomoea nil.

Authors: Hoshino, Atsushi and Jayakumar, Vasanthan and Nitasaka, Eiji and Toyoda, Atsushi and Noguchi, Hideki and Itoh, Takehiko and Shin-I, Tadasu and Minakuchi, Yohei and Koda, Yuki and Nagano, Atsushi J and Yasugi, Masaki and Honjo, Mie N and Kudoh, Hiroshi and Seki, Motoaki and Kamiya, Asako and Shiraki, Toshiyuki and Carninci, Piero and Asamizu, Erika and Nishide, Hiroyo and Tanaka, Sachiko and Park, Kyeung-Il and Morita, Yasumasa and Yokoyama, Kohei and Uchiyama, Ikuo and Tanaka, Yoshikazu and Tabata, Satoshi and Shinozaki, Kazuo and Hayashizaki, Yoshihide and Kohara, Yuji and Suzuki, Yutaka and Sugano, Sumio and Fujiyama, Asao and Iida, Shigeru and Sakakibara, Yasubumi

Ipomoea is the largest genus in the family Convolvulaceae. Ipomoea nil (Japanese morning glory) has been utilized as a model plant to study the genetic basis of floricultural traits, with over 1,500 mutant lines. In the present study, we have utilized second- and third-generation-sequencing platforms, and have reported a draft genome of I. nil with a scaffold N50 of 2.88?Mb (contig N50 of 1.87?Mb), covering 98% of the 750?Mb genome. Scaffolds covering 91.42% of the assembly are anchored to 15 pseudo-chromosomes. The draft genome has enabled the identification and cataloguing of the Tpn1 family transposons, known as the major mutagen of I. nil, and analysing the dwarf gene, CONTRACTED, located on the genetic map published in 1956. Comparative genomics has suggested that a whole genome duplication in Convolvulaceae, distinct from the recent Solanaceae event, has occurred after the divergence of the two sister families.

Journal: Nature communications
DOI: 10.1038/ncomms13295
Year: 2016

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