Finished bacterial genomes from shotgun sequence data.

Authors: Ribeiro, Filipe J and Przybylski, Dariusz and Yin, Shuangye and Sharpe, Ted and Gnerre, Sante and Abouelleil, Amr and Berlin, Aaron M and Montmayeur, Anna and Shea, Terrance P and Walker, Bruce J and Young, Sarah K and Russ, Carsten and Nusbaum, Chad and MacCallum, Iain and Jaffe, David B

Exceptionally accurate genome reference sequences have proven to be of great value to microbial researchers. Thus, to date, about 1800 bacterial genome assemblies have been "finished" at great expense with the aid of manual laboratory and computational processes that typically iterate over a period of months or even years. By applying a new laboratory design and new assembly algorithm to 16 samples, we demonstrate that assemblies exceeding finished quality can be obtained from whole-genome shotgun data and automated computation. Cost and time requirements are thus dramatically reduced.

Journal: Genome research
DOI: 10.1101/gr.141515.112
Year: 2012

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