Colistin resistance mediated by mcr-1-harbouring plasmids is an emerging threat in Enterobacteriaceae, like Salmonella. Based on its major contribution to the diarrhoea burden, the epidemic state and threat of mcr-1-harbouring Salmonella in community-acquired infections should be estimated.This retrospective study analysed the mcr-1 gene incidence in Salmonella strains collected from a surveillance on diarrhoeal outpatients in Shanghai Municipality, China, 2006-2016. Molecular characteristics of the mcr-1-positive strains and their plasmids were determined by genome sequencing. The transfer abilities of these plasmids were measured with various conjugation strains, species, and serotypes.Among the 12,053 Salmonella isolates, 37 mcr-1-harbouring strains, in which 35 were serovar Typhimurium, were detected first in 2012 and with increasing frequency after 2015. Most patients infected with mcr-1-harbouring strains were aged <5?years. all strains, including fluoroquinolone-resistant and/or extended-spectrum ß-lactamase-producing were multi-drug resistant. s. typhimurium had higher mcr-1 plasmid acquisition ability compared with other common serovars. phylogeny based on the genomes combined complete sequences revealed some clusters, suggesting presence of mcr-1-harbouring salmonella outbreaks in community. most mcr-1-positive strains clustered together pork strongly consumption as a main infection source.the prevalence community-acquired diarrhoea displays rapid increase trend, and esbl-mcr-1-harbouring poses threat for children. these findings highlight necessary significance prohibiting colistin use animals continuous monitoring salmonella.copyright © 2019. published by elsevier b.v.
Journal: EBioMedicine
DOI: 10.1016/j.ebiom.2019.03.006
Year: 2019