July 7, 2019  |  

Development of new methods for the quantitative detection and typing of Lactobacillus parabuchneri in dairy products

Authors: Berthoud, Hélène and Wüthrich, Daniel and Bruggmann, Rémy and Wechsler, Daniel and Fröhlich-Wyder, Marie-Therese and Irmler, Stefan

Thirty-one isolates of Lactobacillus parabuchneri were obtained from cheese containing histamine; of these, 26 were found to possess the hdcA gene encoding histidine decarboxylase. By analysing the genome data of 13 isolates, specific targets for the development of PCR-based detection and typing systems for L. parabuchneri were identified. The real-time PCR for detection showed a linear quantification over a range of 7 logs and a detection limit of 10 gene equivalents per reaction. The strain typing method utilised the amplification of repeat sequences and showed discrimination comparable with a phylogenetic tree, based on genome comparisons. The method was suitable for detecting and monitoring the development of L. parabuchneri in raw milk and cheese.

Journal: International dairy journal
DOI: 10.1016/j.idairyj.2016.10.005
Year: 2017

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