Recent studies indicate that there is selection bias for transmission of viral polymorphisms associated with higher viral fitness. Furthermore, after transmission and before a specific immune response is mounted in the recipient, the virus undergoes a number of reversions which allow an increase in their replicative capacity. These aspects, and others, affect the viral population characteristic of early acute infection.160 singlegag-gene amplifications were obtained by limiting-dilution RT-PCR from plasma samples of 8 ARV-naïve patients with early acute infection (<30?days, 22?days average) and 8 arv-naive patients with approximately a year of infection (10 amplicons per patient). sanger sequencing ngs smrt technology (pacific biosciences) were implemented to sequence the amplicons. phylogenetic analysis was performed by using mega 6.06. hla-i (a b) typing ssop-pcr method. chromatograms analyzed sequencher 4.10. epitopes immune-proteosomal cleavages prediction cbs server for 30 hla-a -b alleles most prevalent in our population peptide lengths from 14 mer. cytotoxic response iedb resource.after implementing epitope analysis, we identified total number 325 possible viral present two or more acute chronic patients. 60.3% (n?=?196) them only (prevalent epitopes) while 39.7% epitopes). within p24, difference equally dramatic 59.4% (79/133) being (p?
Journal: Vaccine
DOI: 10.1016/j.vaccine.2018.04.086
Year: 2018