July 19, 2019  |  

Advantages of Single-Molecule Real-Time Sequencing in high-GC content genomes.

Authors: Shin, Seung Chul and Ahn, Do Hwan and Kim, Su Jin and Lee, Hyoungseok and Oh, Tae-Jin and Lee, Jong Eun and Park, Hyun

Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution.

Journal: PloS one
DOI: 10.1371/journal.pone.0068824
Year: 2013

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