June 1, 2021

Whole gene sequencing of KIR-3DL1 with SMRT Sequencing and the distribution of allelic variants in different ethnic groups

Author(s): Cereb, N and Kim, H and Ryu, J and Kim, E and Rai, V, and Choi, S and Vierra-Green, C and Spellman, S and Jeon, J and Kwon, J and Yang, S. Y.

The killer-cell immunoglobulin-like receptor (KIR) gene family are involved in immune modulation during viral infection, autoimmune disease and in allogeneic stem cell transplantation. Most KIR gene diversity studies and their impact on the transplant outcome is performed by gene absence/presence assays. However, it is well known that KIR gene allelic variations have biological significance. Allele level typing of KIR genes has been very challenging until recently due to the homologous nature of those genes and very long intronic sequences. SMRT (Single Molecule Real-Time) Sequencing generates average long reads of 10 to 15 kb and allows us to obtain in-phase long sequence reads. We have developed a PCR assay for SMRT Sequencing on the PacBio RS II platform in our lab for 3DL1 whole gene sequencing. This approach allows us to obtain allele level typing for 3DL1 genes and could serve as a model to type other KIR genes at allelic level.

Organization: Histogenetics
Year: 2016

View Conference Poster

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.