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Sequence previously
unsequenceable regions

Enrichment of hard-to-amplify genomic regions like repeat expansions is now possible with our no-amplification (No-Amp) targeted sequencing method utilizing the CRISPR/Cas9 system.

Unraveling Repeat Expansion Disorders

Many of the disease-causative genes for repeat expansion disorders were mapped decades ago; however, the underlying disease mechanisms are still not fully understood. These expansions can be several kilobases in size which makes them inaccessible with base-level resolution to most technologies. Now, by combining No-Amp targeted enrichment using the CRISPR/Cas9 system with SMRT Sequencing, scientists can holistically in one experiment:

  • Eliminate PCR bias and errors
  • Sequence through entire repeat expansions with base-level resolution
  • Quantify repeat numbers in normal- and mutant-expanded alleles
  • Identify interruption sequences
  • Characterize somatic mosaicism

Watch this short video to learn how No-Amp targeted sequencing works

 

Workflow: from gDNA to Complete Repeat Expansion Sequence


Sample and Library Prep
With our 2-day No-Amp protocol you can now enrich for your region of interest without  amplification using the CRISPR/Cas9 system.

Learn More


Sequencing
Use the Sequel Systems to accurately sequence a human genome.

Learn More


Data Analysis
Analysis solutions for every user in the lab with SMRT Link and visualization tools.

Learn More


 

Spotlight: No-Amp targeted sequencing advances Ataxia research

Scientists are exploring the genetic composition of complete repeat expansions to uncover novel phenotype-genotype correlations between Parkinson’s disease and the gene ATXN10. Explore this research further.

To learn more about No-Amp targeted sequencing, contact us.

Selected Resources