May 10, 2024  |  Educational video

Understanding full-length single-cell RNA-seq of immune cells in cardiovascular diseases

Discover how full-length isoform sequencing enables you to robustly and cost effectively identify new isoforms at scale and study isoform dynamics in single-cell populations. The MAS-Seq for 10x Single Cell 3’ kit – and its successor, the Kinnex single-cell RNA kit – concatenate isoforms for highly accurate long-read sequencing (HiFi sequencing). This approach increases throughput and reduces costs by giving you a 16-fold throughput increase compared to un-concatenated libraries, without losing accuracy or full-length isoform information. Anastasiya Grinko, MSc, applied the MAS-Seq kit to profile the single-cell isoform landscape of monocyte-derived macrophages after myocardial infarction. This approach captured thousands of distinct isoforms, making it possible to see cell-type-specific isoform utilization in monocytes and macrophage subtypes and to unveil temporal dynamics of isoform expression. Watch this webinar to: • Understand the importance of elucidating isoforms in single-cell transcriptomics. • Learn how to analyze MAS-Seq and Kinnex single-cell data with the Single-cell Iso-Seq workflow in SMRT Link. • See how the throughput of the MAS-Seq single-cell kit revealed differentially expressed isoforms across cell types in heart tissue after myocardial infarction.

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