Delivering a more complete view of genetic diversity
A deeper understanding of the genetics underlying biological traits is a key factor in resolving many agricultural, industrial, and environmental challenges.
The PacBio Systems are suited for a wide range of applications and provide scientists with the ability to:
Explore evolution and diversity in high resolution
Often only a single, fragmented reference genome is available for a species, genus, or even family, limiting discovery potential. Past use of short, biased next-generation reads often resulted in poor draft-quality genomes. High-quality reference genomes with evidence-based gene annotations deliver the most complete view of genetic diversity, allowing scientists to make novel and meaningful discoveries.
Uncover key genes for improved agricultural products
Whether breeding crops or livestock, preventing and treating disease, or protecting against pests, a deeper understanding of genetic components is critical for designing high-performance products. High-throughput, long-read, and cost-effective sequencing produces the most complete and accurate view of genes of interest, enabling a faster response to market demands.
Engineer organisms with precision for industrial applications
By optimizing existing biochemical pathways or engineering new ones, scientists turn microbes and plants into safe, contained manufacturing centers. SMRT Sequencing delivers a seamless process for sequencing individual organisms or parsing out microbial communities, resulting in complete and accurate genome assemblies as a basis for engineering.
SMRT Sequencing Project Calculators
Tell us about your plant or animal genome of interest and receive an estimate on the time and materials needed using the Sequel System for:
- Whole genome sequencing for de novo assembly
- Iso-Seq method for full-length transcript isoforms
Spotlight: Closing Gaps, Advancing Science
A new article in Science highlights how PacBio sequencing is improving plant and animal genomes. Explore this research further.
Pennisi, E., 2017. New technologies boost genome quality. Science. Jul 7;357(6346):10-11.
Selected Resources
- Workman, Rachael E et al. (2018) Single molecule, full-length transcript sequencing provides insight into the extreme metabolism of ruby-throated hummingbird Archilochus colubris GigaScience
- Nowoshilow, Sergej et al. (2018) The axolotl genome and the evolution of key tissue formation regulators. Nature
- Makita, Yuko et al. (2018) Construction of Pará rubber tree genome and multi-transcriptome database accelerates rubber researches. BMC Genomics
- Fallon, Timothy R et al. (2017) Firefly genomes illuminate the origin and evolution of bioluminescence bioRxiv
- Beaulaurier, John et al. (2017) Metagenomic binning and association of plasmids with bacterial host genomes using DNA methylation. Nature Biotechnology
- Lu, You et al. (2017) Comparative genomic analyses of Clavibacter michiganensis subsp. insidiosus and pathogenicity on Medicago truncatula. Phytopathology
- Hobbs, Matthew et al. (2017) Long-read genome sequence assembly provides insight into ongoing retroviral invasion of the koala germline. Scientific Reports
- Zhao, Guangyao et al. (2017) The Aegilops tauschii genome reveals multiple impacts of transposons Nature Plants
- Luo, Ming-Cheng et al. (2017) Genome sequence of the progenitor of the wheat D genome Aegilops tauschii. Nature
- Semler, Matthew R et al. (2017) Novel full-length major histocompatibility complex class I allele discovery and haplotype definition in pig-tailed macaques. Immunogenetics
- Verhey, Theodore B et al. (2017) Analysis of recombinational switching at the antigenic variation locus of the Lyme spirochete using a novel PacBio sequencing pipeline. Molecular Microbiology
- Jayakumar, Vasanthan et al. (2017) Comprehensive evaluation of non-hybrid genome assembly tools for third-generation PacBio long-read sequence data. Briefings in Bioinformatics
- Ichikawa, Kazuki et al. (2017) Centromere evolution and CpG methylation during vertebrate speciation. Nature Communications
- Cheng, Bing et al. (2017) Long-read sequencing of the coffee bean transcriptome reveals the diversity of full-length transcripts. GigaScience
- Zimin, Aleksey V et al. (2017) The first near-complete assembly of the hexaploid bread wheat genome, Triticum aestivum. GigaScience
- Rödelsperger, Christian et al. (2017) Single-molecule sequencing reveals the chromosome-scale genomic architecture of the nematode model organism Pristionchus pacificus. Cell Reports
- Teh, Bin Tean et al. (2017) The draft genome of tropical fruit durian (Durio zibethinus). Nature Genetics
- Fraiture, Marie-Alice et al. (2017) An integrated strategy combining DNA walking and NGS to detect GMOs. Food Chemistry
- Zhang, Shi-Jian et al. (2017) Isoform evolution in primates through independent combination of alternative RNA processing events. Molecular Biology and Evolution
- Wooddell, Christine I et al. (2017) RNAi-based treatment of chronically infected patients and chimpanzees reveals that integrated hepatitis B virus DNA is a source of HBsAg. Science Translational Medicine
- Sayadi, Ahmed et al. (2017) The evolution of dark matter in the mitogenome of seed beetles. Genome Biology and Evolution
- Edwards, Joan E et al. (2017) PCR and omics based techniques to study the diversity, ecology and biology of anaerobic fungi: Insights, challenges andopportunities. Frontiers in Microbiology
- Li, Yongping et al. (2017) Genome re-annotation of the wild strawberry Fragaria vesca using extensive Illumina-and SMRT-based RNA-seq datasets DNA Research
- Bartling, Pascal et al. (2017) The composite 259-kb plasmid of Martelella mediterranea DSM 17316(T)-a natural replicon with functional RepABC modules from Rhodobacteraceae and Rhizobiaceae. Frontiers in Microbiology
- Hebert, Paul DN et al. (2017) A sequel to Sanger: amplicon sequencing that scales bioRxiv
- Wang, Maojun et al. (2017) A global survey of alternative splicing in allopolyploid cotton: landscape, complexity and regulation. The New Phytologist
- Dallery, Jean-Félix et al. (2017) Gapless genome assembly of Colletotrichum higginsianum reveals chromosome structure and association of transposable elements with secondary metabolite gene clusters. BMC Genomics
- Li, Jun et al. (2017) Long read reference genome-free reconstruction of a full-length transcriptome from Astragalus membranaceus reveals transcript variants involved in bioactive compound biosynthesis. Cell Discovery
- Korlach, Jonas et al. (2017) De novo PacBio long-read and phased avian genome assemblies correct and add to reference genes generated with intermediate and short reads. GigaScience
- van Dam, Peter et al. (2017) A mobile pathogenicity chromosome in Fusarium oxysporum for infection of multiple cucurbit species. Scientific Reports
- Bao, Jiandong et al. (2017) PacBio sequencing reveals transposable element as a key contributor to genomic plasticity and virulence variation in Magnaporthe oryzae. Molecular Plant
- Pei, Weike et al. (2017) Polylox barcoding reveals haematopoietic stem cell fates realized in vivo. Nature
- Nudelman, German et al. (2017) High resolution annotation of zebrafish transcriptome using long-read sequencing bioRxiv
- Chen, Shi-Yi et al. (2017) A transcriptome atlas of rabbit revealed by PacBio single-molecule long-read sequencing. Scientific Reports
- Gao, Shan et al. (2017) Two novel lncRNAs discovered in human mitochondrial DNA using PacBio full-length transcriptome data. Mitochondrion
- Poster: Vierra, Michelle et al. (2018) Full-length transcript profiling with the Iso-Seq method for improved genome annotations
- Poster: Korlach, Jonas et al. (2018) High-quality de novo genome assembly and intra-individual mitochondrial instability in the critically endangered kakapo
- Poster: Heiner, Cheryl et al. (2018) Multiplexed Complete Microbial Genomes on the Sequel System
- Poster: Kingan, S., et al. (2018) Best practices for diploid assembly of complex genomes using PacBio: A case study of Cascade Hops
- Poster: Concepion, C., et al. (2018) De novo assembly and preliminary annotation of the Schizocardium californicum genome
- Poster: Tseng, E., et al. (2018) Haplotyping of full-length transcript reads from long-read sequencing can reveal allelic imbalances in isoform expression
- Poster: Concepcion, G., et al. (2018) Structural variant detection in crops using PacBio SMRT Sequencing
- Poster: Vierra, M. et al. (2017) From RNA to full-length transcripts: The PacBio Iso-Seq method for transcriptome analysis and genome annotation
- Poster: Kingan, Sarah et al. (2017) A high-quality genome assembly of SMRT sequences reveals long range haplotype structure in the diploid mosquito Aedes aegypti
- Poster: Korlach, Jonas et al. (2017) De novo PacBio long-read assembled avian genomes correct and add to genes important in neuroscience and conservation research
- Poster: Kingan, S. et al. (2017) A high-quality genome assembly of SMRT Sequences reveals long-range haplotype structure in the diploid mosquito Aedes aegypti
- Poster: Sisneros, N. et al. (2017) Best practices for whole genome sequencing Using the Sequel System
- Poster: Warr, A. et al. (2017) High-quality, highly contiguous re-assembly of the pig genome
- Poster: Concepcion, G. et al. (2017) Phased diploid genome assembly with single-molecule real-time sequencing
- Poster: Heiner, C. et al. (2017) Profiling complex population genomes with highly accurate single molecule reads: cow rumen microbiomes
- Poster: Clark, T et al. (2017) Full-length cDNA sequencing on the PacBio Sequel platform
- Poster: Roach, M. et al. (2016) Assessing diversity and clonal variation of Australia’s grapevine germplasm: Curating the FALCON-Unzip Chardonnay de novo genome assembly
- Poster: Tseng, Elizabeth et al. (2016) Reconstruction of the spinach coding genome using full-length transcriptome without a reference genome
- Poster: Baybayan, Primo et al. (2016) Application specific barcoding strategies for SMRT Sequencing
- Poster: Ranade, Swati et al. (2016) Immune regions are no longer incomprehensible with SMRT Sequencing
- Poster: Seetin, Matthew et al. (2016) Long-read assembly of the Aedes aegypti Aag2 cell line genome resolves ancient endogenous viral elements
- Poster: Heiner, Cheryl et al. (2016) Low-input long-read sequencing for complete microbial genomes and metagenomic community analysis
- Poster: Chin, Jason et al. (2016) Un-zipping diploid genomes – revealing all kinds of heterozygous variants from comprehensive hapltotig assemblies
- Presentation: Chin, Jason et al. (2016) Diploid genome assembly and comprehensive haplotype sequence reconstruction
- Poster: Clark, Tyson et al. (2016) Full-length cDNA sequencing for genome annotation and analysis of alternative splicing
- Poster: Seetin, Matthew G et al. (2016) Long-read assembly of the Aedes aegypti Aag2 cell line genome resolves ancient endogenous viral elements
- Poster: Hall, Richard J et al. (2016) Profiling metagenomic communities using circular consensus and Single Molecule, Real-Time Sequencing
- Poster: Muehling, Jill R et al. (2016) SMRT Sequencing of the alala genome
- Poster: Lee, Hayan et al. (2015) The resurgence of reference quality genome
- Poster: Rank, David et al. (2015) Best practices for whole-genome de novo sequencing with long-read SMRT Sequencing.
- Presentation: Schatz, Michael et al. (2015) The resurgence of reference quality genome sequence.
- Presentation: VanBuren, Robert et al. (2015) De novo assembly of a complex panicoid grass genome using ultra-long PacBio reads with P6C4 chemistry
- Presentation: Strickler, Susan R et al. (2015) Genome assembly strategies of the recent polyploid, Coffea arabica.
- Presentation: Wing, Rod A et al. (2015) Old school/new school genome sequencing: One step backward — a quantum leap forward.
- Poster: Copetti, Dario et al. (2015) Rapid full-length Iso-Seq cDNA sequencing of rice mRNA to facilitate annotation and identify splice-site variation.
- Ballard, Bill (2018) PAG Conference: Domestication: through the canines of a dingo
- Renner, Tanya (2018) PAG Conference: Long-read sequencing reveals complex genomic architecture in independent carnivorous plant lineages
- Williams, John (2018) PAG Conference: Using cattle subspecies crosses to explore chromosome of origin expression through Iso-seq analysis
- Hatas, Emily (2017) Webinar: SMRT Sequencing applications in plant and animal sciences: an overview
- Kujawa, Steve (2017) Webinar: PacBio Targeted Sequencing of Long Amplicons Using PCR or Hybrid Capture
- Turner, Stephen (2017) Webinar: An Introduction to PacBio’s Long-Read Sequencing & How It Has Been Used to Make Important Scientific Discoveries
- Neafsey, Daniel (2017) AGBT Conference: A community effort using multiple technologies to produce a dramatically improved genome assembly of the Zika virus mosquito vector
- Roy, Margaret (2017) AGBT PacBio Workshop: De novo sequencing of the naked mole rat genome
- Korlach, Jonas (2017) AGBT PacBio Workshop: SMRT Sequencing roadmap: better throughput, lower costs
- Sedlazeck, Fritz (2017) AGBT Virtual Poster: Quantifying the impact of improved genome assemblies on functional genomics
- Simon, Matt (2017) Video: Get ready for super coffee strains. Scientists just sequenced the plant’s DNA
- (2017) Tutorial: Iso-Seq analysis application
- (2017) Tutorial: Long Amplicon Analysis application
- Jarvis, Erich (2017) PAG PacBio Workshop: Comparative analyses of next generation technologies for generating chromosome-level reference genome assemblies
- Johnson, Rebecca (2017) PAG PacBio Workshop: De novo sequencing of the koala genome
- Matthews, Ben (2017) PAG PacBio Workshop: Genome assembly and molecular genetics of the dengue, yellow fever, and zika vector Aedes aegypti
- Wing, Rod (2017) PAG PacBio Workshop: Introducing 5 new high-quality PacBio genome assemblies for rice to help solve the 10-billion people question
- Korlach, Jonas (2017) PAG PacBio Workshop: SMRT Sequencing for complete genomes
- Kuo, Richard (2017) PAG PacBio Workshop: Using the Iso-Seq method to fill in your a_no__tion
- Korlach, J. and Kingan, S. (2016) i5K Webinar: High-quality de novo insect genome assemblies using PacBio sequencing
- Hannigan, Brett (2016) DNAnexus Webinar: Simplifying de novo assembly with PacBio tools available on DNAnexus: FALCON
- Hill, Christopher (2016) AGBT Conference: Long-read sequence of the gorilla genome
- Ranade, Swati (2016) AGBT Virtual Poster: Immune regions are no longer incomprehensible with SMRT Sequencing
- Seetin, Matthew (2016) AGBT Virtual Poster: Long-read assembly of the Aedes aegypti Aag2 cell line genome resolves ancient endogenous viral elements
- Heiner, Cheryl (2016) AGBT Virtual Poster: Low-input long-read sequencing for complete microbial genomes and metagenomic community analysis
- Heiner, Cheryl (2016) AGBT Virtual Poster: Minimization of chimera formation and substitution errors in full-length 16S PCR amplification
- Chin, Jason (2016) AGBT Virtual Poster: Unzipping diploid genomes – revealing all kinds of heterozygous variants from comprehensive haplotig assemblies
- Ware, Doreen (2016) PAG PacBio Workshop: A-maize-ing time for plant science – SMRT Sequencing of the maize genome and transcriptome
- Ryder, Oliver (2016) PAG PacBio Workshop: Conservation genomics of a critically endangered Hawaiian bird: A high quality genome assembly of the ‘alala will assist in population management and reintroduction
- Shwen, Ho Yung (2016) PAG PacBio Workshop: Empowering genetics research in Chenopodium quinoa with single molecule genomics
- Cramer, Grant and Cantu, Dario and Adam Blondon, Anney-Francoise (2016) PAG Conference: Update on sequencing of the Cabernet sauvignon genome
- Timp, Winston (2016) PAG Conference: An extreme metabolism: Iso-Seq analysis of the ruby-throated hummingbird transcriptome
- Smith, Tim (2016) PAG Conference: Approaches taken, progress made, and enhanced utility of long read-based goat, swine, cattle and sheep reference genomes
- Chin, Jason (2016) PAG Conference: Diploid genome assembly and comprehensive haplotype sequence reconstruction
- Berges, Hélène (2016) PAG Conference: Long reads sequencing technology to solve complex genomic regions assembly in plants
- Application Brief: Long-read RNA sequencing – Best Practices. (2018)
- Application Brief: Large genome whole genome sequencing – Best Practices. (2018)
- De Novo Assembly Brochure: Assembly Options for your SMRT Sequencing Data (2018)
- SMRT Sequencing Brochure: Revolutionize genomics with SMRT Sequencing. (2018)
- Case Study: Pioneering a pan-genome reference collection (2018)
- PacBio Certified Service Providers (2018)
- Case Study: SMRT sequencing brings clarity to HIV vaccine and transplant research at the Wisconsin national primate research center. (2017)
- Plant and Animal Sciences Brochure: A comprehensive view of genetic diversity (2017)
- Case Study: PacBio and Dovetail – For cashew genome, combining technologies yields chromosome-scale assembly. (2017)
- Case Study: Breeding a better Cabernet – SMRT Sequencing provides detailed view of grape genome. (2016)
- Application Note: Multiplex target enrichment using barcoded multi-kilobase fragments and probe-based capture technologies. (2016)
- Application Note: Targeted sequencing and chromosomal haplotype assembly using Cergentis TLA technology with SMRT Sequencing. (2016)
- Case Study: First comprehensive view of maize genome reveals regulatory and structural mechanisms. (2016)
- Case Study: With SMRT Sequencing for genomes, transcriptomes, and epigenomes, scientists are overcoming barriers in plant and animal research. (2015)
- Certified Service Provider Profile: AGI builds on BAC expertise and SMRT Sequencing for rice and other crop genomes. (2015)
- Targeted Sequencing Application Brochure: Capture your regions of interest in high resolution. (2015)
- Case Study: Scientists create gold standard plant and animal genomes with SMRT Sequencing. (2015)