Sequence plants and animals with confidence
Large and complex genomes present unique challenges such as polyploidy and high levels of heterozygosity. PacBio offers the long reads with high accuracy necessary to overcome these challenges.
Insect Biology
Insect Biology Develop disease prevention methods, understand basic biology, and improve pest control using a population or a single individual.
Agricultural pest rapidly sequenced | Genome of the ice worm, a glacier dwelling annelid | Alternative splicing profiling in diamondback moth
Crop Improvements
Crop ImprovementsUncover new biology to improve crop health, conduct trait selection, and enhance breeding efficiency.
Ginkgo biloba transcript variants in flavonoid biosynthesis | Tracing plant immunity with resistance genes | Smoking out structural variants in the cannabis genome
Animal Health and Breeding
Animal Health and BreedingInvestigate animal genetics to study patterns of inheritance, immunology, and domestication in high resolution.
Structural variation and adaptation in cattle | Grizzly bears and the biomedical promise of hibernation | Studying fibrotic myopathy in German Shepherds
Marine Biodiversity
Marine BiodiversityDive into the depths of marine ecosystems to study evolution, reveal insights into developmental and address the challenges of climate change.
Revealing genetic mysteries of marine life | Isoform sequencing to ward off disease in shrimp | Exploring crustacean genomes for viruses
Conservation
ConservationExamine the genetic diversity of species-at-risk to support assisted reproduction, wildlife management, and sustainability.
Sequencing the California redwood | Consortium releases 100 high-quality genomes | International Barcode of Life project to monitor life
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Whole Genome Sequencing Produce reference-quality genomes with phased haplotypes from even small-bodied organisms |
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Structural Variant Calling Use high-sensitivity variant calls with low false discovery rate to gain actionable insights across populations |
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Targeted Sequencing Choose from flexible options to target regions to access to all variants even in the most complex regions |
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RNA Sequencing Access full-length transcript sequences to identify novel genes and transcripts and improve genome annotation |
2020 HiFi for All – Collaborations SMRT Grant
Sequence All Life with Confidence
Apply by January 22, 2021 for your chance to win free HiFi sequencing for collaborative teams of up to 10 scientists.
Selected Resources
- Grech-Baran, Marta et al. (2020) Extreme resistance to Potato virus Y in potato carrying the Rysto gene is mediated by a TIR-NLR immune receptor. Plant biotechnology journal
- Ye, Jiabao et al. (2019) A global survey of full-length transcriptome of Ginkgo biloba reveals transcript variants involved in flavonoid biosynthesis Industrial Crops and Products
- Loit, Kaire et al. (2019) Relative Performance of MinION (Oxford Nanopore Technologies) versus Sequel (Pacific Biosciences) Third-Generation Sequencing Instruments in Identification of Agricultural and Forest Fungal Pathogens. Applied and environmental microbiology
- Kingan, Sarah B et al. (2019) A high-quality genome assembly from a single, field-collected spotted lanternfly (Lycorma delicatula) using the PacBio Sequel II system GigaScience
- Tørresen, Ole K et al. (2019) Tandem repeats lead to sequence assembly errors and impose multi-level challenges for genome and protein databases. Nucleic acids research
- He, Yaoxi et al. (2019) Long-read assembly of the Chinese rhesus macaque genome and identification of ape-specific structural variants. Nature communications
- Zhou, Yongfeng et al. (2019) The population genetics of structural variants in grapevine domestication. Nature plants
- Meier, Matthew J et al. (2019) Whole Genome Sequencing of the Mutamouse Model Reveals Strain- and Colony-Level Variation, and Genomic Features of the Transgene Integration Site. Scientific reports
- Wenger, Aaron M et al. (2019) Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome. Nature biotechnology
- Liu, Qin et al. (2019) A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata. GigaScience
- Watson, Corey T et al. (2019) A comparison of immunoglobulin IGHV, IGHD and IGHJ genes in wild-derived and classical inbred mouse strains. Immunology and cell biology
- Van de Weyer, Anna-Lena et al. (2019) A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana. Cell
- Bickhart, Derek M et al. (2019) Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long-read assembly and proximity ligation. Genome biology
- Jaworski, Coline C et al. (2019) Chromosome-level hybrid de novo genome assemblies as an attainable option for non-model organisms BioRxiv
- Low, Wai Yee et al. (2019) Haplotype-Resolved Cattle Genomes Provide Insights Into Structural Variation and Adaptation BioRxiv
- Martijn, Joran et al. (2019) Confident phylogenetic identification of uncultured prokaryotes through long read amplicon sequencing of the 16S-ITS-23S rRNA operon. Environmental microbiology
- Yang, Zhaoen et al. (2019) Extensive intraspecific gene order and gene structural variations in upland cotton cultivars. Nature communications
- Novák, Karel et al. (2019) Potential of TLR-gene diversity in Czech indigenous cattle for resistance breeding as revealed by hybrid sequencing Archives Animal Breeding
- Wan, Ying et al. (2019) Systematic identification of intergenic long-noncoding RNAs in mouse retinas using full-length isoform sequencing. BMC genomics
- McCartney, Michael A. et al. (2019) The Genome of the Zebra Mussel, Dreissena polymorpha: A Resource for Invasive Species Research BioRxiv
- Zan, Jindong et al. (2019) A microbial factory for defensive kahalalides in a tripartite marine symbiosis. Science
- Warr, Amanda et al. (2019) An improved pig reference genome sequence to enable pig genetics and genomics research BioRxiv
- Chen, Zelin et al. (2019) De novo assembly of the goldfish (Carassius auratus) genome and the evolution of genes after whole-genome duplication. Science advances
- Yang, Ning et al. (2019) Genome assembly of a tropical maize inbred line provides insights into structural variation and crop improvement. Nature genetics
- Sánchez-Pérez, R et al. (2019) Mutation of a bHLH transcription factor allowed almond domestication. Science
- Yoshimura, Jun et al. (2019) Recompleting the Caenorhabditis elegans genome. Genome research
- Tedersoo, Leho et al. (2019) Towards PacBio-based pan-eukaryote metabarcoding using full-length ITS sequences. Environmental microbiology reports
- Wang, Bo et al. (2019) Variant Phasing and Haplotypic Expression from Single-molecule Long-read Sequencing in Maize BioRxiv
- Zhang, Peng et al. (2019) Full-Length Multi-Barcoding: DNA Barcoding from Single Ingredient to Complex Mixtures. Genes
- Chang, Ching-Ho et al. (2019) Islands of retroelements are major components of Drosophila centromeres PLoS biology
- Jamy, Mahwash et al. (2019) Long metabarcoding of the eukaryotic rDNA operon to phylogenetically and taxonomically resolve environmental diversity BioRxiv
- Kao, Tzu-Tong et al. (2019) Low-copy nuclear sequence data confirm complex patterns of farina evolution in notholaenid ferns (Pteridaceae). Molecular phylogenetics and evolution
- Zhuang, Weijian et al. (2019) The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication. Nature genetics
- Hotaling, Scott et al. (2019) The rising tide of high-quality genomic resources. Molecular ecology resources
- Kronenberg, Zev N. et al. (2019) Extended haplotype phasing of de novo genome assemblies with FALCON-Phase BioRxiv
- Poster: Baybayan, Primo et al. (2020) New advances in SMRT Sequencing facilitate multiplexing for de novo and structural variant studies
- Poster: Tseng, Elizabeth et al. (2020) A complete solution for high-quality genome annotation using the PacBio Iso-Seq method
- Poster: Galvin, Brendan et al. (2020) A high-quality PacBio insect genome from 5 ng of input DNA
- Poster: Kingan, Sarah B. et al. (2020) Beyond Contiguity: Evaluating the accuracy of de novo genome assemblies
- Poster: Kingan, Sarah B. et al. (2020) Every species can be a model: Reference-quality PacBio genomes from single insects
- Poster: Vierra, M. et al. (2019) Structural variant detection in crops using low-fold coverage long-read sequencing
- Poster: Duncan, Turner et al. (2019) A low DNA input protocol for high-quality PacBio de novo genome assemblies
- Poster: Ekholm, Jenny et al. (2019) Streamlines SMRTbell library generation using addition-only, single tube strategy for all library types reduces time to results
- Poster: Baybayan, Primo et al. (2019) A high-quality de novo genome assembly from a single mosquito using PacBio sequencing
- Poster: Kingan, Sarah B. et al. (2019) A low DNA input protocol for high-quality PacBio de novo genome assemblies from single invertebrate individuals
- Poster: Tseng, Elizabeth et al. (2019) Haplotyping using full-length transcript sequencing reveals allele-specific expression
- Poster: Vierra, Michelle et al. (2019) Library prep and bioinformatics improvements for full-length transcript sequencing on the PacBio Sequel System
- Poster: Peluso, Paul et al. (2019) Single molecule high-fidelity (HiFi) Sequencing with >10 kb libraries
- Poster: Blethrow, J. Sisneros, N. et al. (2018) Best practices for whole genome sequencing using the Sequel System
- Poster: Vierra, Michelle et al. (2018) Full-length transcript profiling with the Iso-Seq method for improved genome annotations
- Poster: Korlach, Jonas et al. (2018) High-quality de novo genome assembly and intra-individual mitochondrial instability in the critically endangered kakapo
- Poster: Heiner, Cheryl et al. (2018) Multiplexed complete microbial genomes on the Sequel System
- Poster: Kingan, S., et al. (2018) Best practices for diploid assembly of complex genomes using PacBio: A case study of Cascade Hops
- Poster: Vierra, Michelle et al. (2018) Characterizing the pan-genome of maize with PacBio SMRT Sequencing
- Poster: Concepion, C., et al. (2018) De novo assembly and preliminary annotation of the Schizocardium californicum genome
- Poster: Tseng, E., et al. (2018) Haplotyping of full-length transcript reads from long-read sequencing can reveal allelic imbalances in isoform expression
- Poster: Concepcion, G., et al. (2018) Structural variant detection in crops using PacBio SMRT Sequencing
- Poster: Vierra, M. et al. (2017) From RNA to full-length transcripts: The PacBio Iso-Seq method for transcriptome analysis and genome annotation
- Poster: Kingan, Sarah et al. (2017) A high-quality genome assembly of SMRT sequences reveals long range haplotype structure in the diploid mosquito Aedes aegypti
- Poster: Korlach, Jonas et al. (2017) De novo PacBio long-read assembled avian genomes correct and add to genes important in neuroscience and conservation research
- Poster: Kingan, S. et al. (2017) A high-quality genome assembly of SMRT Sequences reveals long-range haplotype structure in the diploid mosquito Aedes aegypti
- Poster: Warr, A. et al. (2017) High-quality, highly contiguous re-assembly of the pig genome
- Poster: Concepcion, G. et al. (2017) Phased diploid genome assembly with single-molecule real-time sequencing
- Poster: Heiner, C. et al. (2017) Profiling complex population genomes with highly accurate single molecule reads: cow rumen microbiomes
- Poster: Clark, T et al. (2017) Full-length cDNA sequencing on the PacBio Sequel platform
- Balacco, Jennifer (2020) Virtual Global Summit: Adventures towards error-free genomes
- Lawniczak, Mara (2020) Virtual Global Summit: Anopheles and the journey from a reference genome to a thousand genomes to spatiotemporal genomic observatories
- Carbonell, Pablo (2020) Virtual Global Summit: Elucidating the origin of grapevine agronomic traits with PacBio genome assembly and Iso-Seq analysis
- Schmutz, Jeremy (2020) Virtual Global Summit: HiFi – Amazing Applications in Plant Genomes and Human Disease Research
- Chong, Tang (2020) Virtual Global Summit: HIT-scISOseq – High-throughput and high-accuracy single-cell full-length isoform sequencing
- Newell, Nicole (2020) Virtual Global Summit: Technical Tutorial – Choosing the best library prep for HiFi sequencing
- Hernandez, Alvaro (2020) Virtual Global Summit: Using the unique capabilities of the Sequel II System for de novo genome assembly and full-length 16S sequencing
- (2020) Video: HiFi Sequencing – Unlock your next great discovery
- Weissensteiner, Matthias and Fengler, Kevin and Mars, Kristin (2020) Webinar: Beyond a single reference genome – The advantages of sequencing multiple individuals
- Guarco, Carmen and Hernandez, Álvaro G. (2020) Spanish Language Webinar: Introducción a la secuenciación larga y precisa con PacBio
- Wenger, Aaron (2020) Educational Video: Variant Detection with HiFi Reads – Understanding Results from the precisionFDA Truth Challenge
- Geib, Scott and Laumer, Christopher and Uliano da Silva, Marcela and Vierra, Michelle (2020) Webinar: No Organism Too Small – Build high-quality genome assemblies of small organisms with HiFi sequencing
- Sovic, Ivan (2020) SMRT Leiden: Improved phased assembly using HiFi data
- Kuo, Richard (2020) SMRT Leiden: Iso-Seq Analysis and beyond! How non-standard analyses of Iso-Seq data can provide insights into your species
- Uliano da Silva, Marcela (2020) SMRT Leiden: The impact of polishing on Darwin Tree of Life chromosome-level lepidopteran genome assemblies built with PacBio HiFi and Illumina Hi-C reads
- Jarvis, Erich (2020) SMRT Leiden: The Vertebrate genomes project: Sequencing life for a new biology
- Datema, Erwin (2020) SMRT Leiden: Ultra-fast comparative genomics of complex genomes at population scale
- Korlach, Jonas and Graves-Lindsay, Tina and Vierra, Michelle (2020) PacBio Workshop: Understanding the biology of genomes with HiFi sequencing
- Kuo, Richard (2020) Customer Experience: A new view of non-coding RNA with PacBio HiFi reads
- Gharbi, Karim (2020) Customer Experience: Advancing UK genomics research with the PacBio Sequel II System
- Schmutz, Jeremy and Conesa, Ana and Gharbi, Karim and Kuo, Richard (2020) Customer Experience: HiFi reads help scientists explore genomes and transcriptomes
- Conesa, Ana (2020) Customer Experience: Improving transcriptome research with PacBio HiFi reads
- Schmutz, Jeremy (2020) Customer Experience: PacBio HiFi reads improve genome sequencing at HudsonAlpha
- Korlach, Jonas (2020) Webinar: Long HiFi reads for high-quality genome assemblies
- Weber, Kay (2020) Webinar: Bioinformatics lunch & learn – HiFi assembly
- Mars, Kristin and Laird Smith, Melissa and Vinnere Pettersson, Olga and Hardigan, Michael (2020) Webinar: Sequencing 101 – How long-read sequencing improves access to genetic information
- Oppert, Brenda (2020) AGBT Presentation: Feed the World – Developing genomic resources for insects as food
- (2020) Video: Introduction to PacBio highly accurate long-read sequencing
- Kronenberg, Zev (2020) PAG Conference: Characterizing genetic differences between great apes using Iso-Seq data
- Blaxter, Mark (2020) PAG Conference: Endless forms: Genomes from the Darwin Tree of Life Project
- Vierra, Michelle (2020) PAG Conference: PacBio update on products and HiFi applications
- U’Ren, Jana (2020) PAG Conference: Phylogenetic insights into the endophyte symbiosis using PacBio ribosomal DNA sequencing
- Nijland, Bart (2020) PAG Conference: The impact of highly accurate PacBio sequence data on the assembly of a tetraploid rose
- Korlach, Jonas (2020) PAG Conference: Workshop introduction
- Sheynkman, Gloria, and Tseng, Elizabeth (2019) Webinar: Discover full-length RNA sequencing – No assembly required
- SMRT Sequencing Brochure: Delivering highly accurate long reads to drive discovery in life science (2020)
- Application Brief: Single-cell RNA sequencing with HiFi reads – Best Practices (2020)
- Application Brochure: What can you do with one SMRT Cell? (2020)
- Product Brochure: Sequel IIe System – Sequencing evolved (2020)
- Informational Guide: Understanding accuracy in DNA sequencing (2020)
- Application Note: Considerations for using the low and ultra-low DNA input workflows for whole genome sequencing (2020)
- Application Brochure: HiFi reads for highly accurate long-read sequencing (2020)
- Technical Note: Preparing DNA for PacBio HiFi sequencing – Extraction and quality control (2020)
- Informational Guide: What’s the value of sequencing full-length RNA transcripts? (2020)
- Informational Guide: Looking beyond the single reference genome to a pangenome for every species (2020)
- Infographic: A Genome Fit for a Giant – Sequencing the California redwood (2020)
- Application Brief: Structural variant detection using whole genome sequencing – Best Practices (2020)
- PacBio Certified Service Providers (2020)
- Application Brief: Whole genome sequencing for de novo assembly – Best Practices (2019)
- Case Study: Diving Deep – Revealing the mysteries of marine life with SMRT Sequencing (2019)
- Application Brief: Targeted sequencing for amplicons – Best Practices (2019)
- Application Brief: Long-read RNA sequencing – Best Practices (2019)
- Infographic: Gold-standard reference genomes accelerate science (2018)
- Technical Note: Preparing samples for PacBio whole genome sequencing for de novo assembly – Collection and storage (2018)
- Case Study: Pioneering a pan-genome reference collection (2018)