Whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of Stenotrophomonas maltophilia bacteremia.

Authors: Pak, Theodore R and Altman, Deena R and Attie, Oliver and Sebra, Robert and Hamula, Camille L and Lewis, Martha and Deikus, Gintaras and Newman, Leah C and Fang, Gang and Hand, Jonathan and Patel, Gopi and Wallach, Fran and Schadt, Eric E and Huprikar, Shirish and van Bakel, Harm and Kasarskis, Andrew and Bashir, Ali

Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Journal: Antimicrobial agents and chemotherapy
DOI: 10.1128/AAC.01723-15
Year: 2015

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